{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:47:04Z","timestamp":1791906424764,"version":"4.3.4"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T00:00:00Z","timestamp":1774915200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Mannheimia haemolytica<\/jats:italic>\n                      is a major bacterial pathogen associated with respiratory diseases in domestic ruminants, resulting in devastating economic losses in the global food production industry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aimed to isolate and assess the antibiotic resistance\/sensitivity patterns of\n                      <jats:italic>M. haemolytica<\/jats:italic>\n                      associated with pneumonic pasteurellosis in sheep in the western Gojjam and Awi zones of Northwest Ethiopia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>One hundred and forty nasal swabs from sheep were collected using purposive sampling and subjected to standard bacteriological and polymerase chain reaction (PCR) assays. The antibiotic susceptibility of the isolates was assessed using Kirby\u2013Bauer's disk diffusion method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The findings of this study revealed that\n                      <jats:italic>M. haemolytica<\/jats:italic>\n                      was the primarily identified agent of pneumonic pasteurellosis in sheep. Molecular analysis confirmed 23\u00a0\n                      <jats:italic>M. haemolytica<\/jats:italic>\n                      isolates. The antibiotic susceptibility test results showed sensitivity to oxytetracycline (78.3%), streptomycin (78.3%), chloramphenicol (87%), kanamycin (100%), gentamicin (100%) and ampicillin (100%), and resistance to erythromycin (100%). Four isolates showed multidrug resistance with a MAR index greater than 0.3.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The findings underscore the potential role of\n                      <jats:italic>M. haemolytica<\/jats:italic>\n                      in causing pneumonic pasteurellosis in sheep in the region. In this study, due to resource limitations, further serotype determination and characterization of antigenic relationships among the isolates were not conducted. Molecular characterization of strains using high\u2010throughput sequencing technologies is needed to elucidate the molecular mechanisms of pathogenesis and develop more effective, widely applicable vaccines and antimicrobial drugs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70897","type":"journal-article","created":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T14:57:11Z","timestamp":1774969031000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection and Antibiotic Susceptibility Profiling of\n                    <i>Mannheimia haemolytica<\/i>\n                    Isolates From Pneumonic Pasteurellosis Suspected Cases of Sheep in Northwestern Ethiopia"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2479-4653","authenticated-orcid":false,"given":"Fanuel Bizuayehu","family":"Yihunie","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine and Animal Sciences Samara University  Samara Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6975-2994","authenticated-orcid":false,"given":"Saddam Mohammed","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Animal Sciences University of Gondar  Gondar Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0802-6638","authenticated-orcid":false,"given":"Destaw Asfaw","family":"Ali","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine and Animal Sciences University of Gondar  Gondar Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1951-2562","authenticated-orcid":false,"given":"Mequanint Addisu","family":"Belete","sequence":"additional","affiliation":[{"name":"Department of Veterinary Laboratory Technology, College of Agriculture and Natural Resources Debre Markos University  Debre Markos Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7026-6267","authenticated-orcid":false,"given":"Teshager","family":"Dubie","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, College of Veterinary Medicine and Animal Sciences Samara University  Samara Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zemenu","family":"Bitew","sequence":"additional","affiliation":[{"name":"Jigjiga University College of Veterinary Medicine  Jigjiga Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takele","family":"Abayneh","sequence":"additional","affiliation":[{"name":"National Veterinary Institute  Bishoftu Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,31]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.1166"},{"key":"e_1_2_12_3_1","first-page":"1","article-title":"Mannheimia haemolytica Infection in Small Ruminants: A Review","volume":"7","author":"Abbas G.","year":"2019","journal-title":"Advances in Zoology and Botany"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2024\/5605552"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/09712119.2022.2146123"},{"key":"e_1_2_12_6_1","first-page":"32","article-title":"Isolation and Identification of Mannhemia and Pasturella Species From Pneumonic and Apparently Healthy Cattle and Their Antibiogram Susceptibility Pattern in Bedelle District, Western Ethiopia","volume":"6","author":"Abera D.","year":"2014","journal-title":"Journal of Bacteriology Research"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.2147\/VMRR.S365267"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.2147\/VMRR.S435932"},{"key":"e_1_2_12_9_1","first-page":"274","article-title":"Epidemiologic and Serologic Investigation of Multifactorial Respiratory Disease of Sheep in the Central Highland of Ethiopia","volume":"2","author":"Ayelet G.","year":"2004","journal-title":"International Journal of Applied Research in Veterinary Medicine"},{"key":"e_1_2_12_10_1","unstructured":"CLSI.2020.Standards for Antimicrobial Susceptibility Testing.CLSI."},{"key":"e_1_2_12_11_1","unstructured":"CSA.2021.Federal Democratic Republic of Ethiopia Central Statistical Agency Agricultural Sample Survey 2020\/21. 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