{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:47:06Z","timestamp":1791906426742,"version":"4.3.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Intensive indoor pig production systems can foster high antimicrobial exposure and dense animal contact, potentially enriching multidrug\u2010resistant (MDR)\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (\n                      <jats:italic>E. coli<\/jats:italic>\n                      ) that may enter wider One Health microbial networks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study investigated grower\u2010finisher Tibetan pigs managed under commercial conditions harbouring phylogenetically diverse\n                      <jats:italic>E. coli<\/jats:italic>\n                      lineages carrying extensive resistance and virulence determinants.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Between 2021 and 2023, 280 rectal swabs were collected from nine mechanically ventilated farms; the isolated\n                      <jats:italic>E.coli<\/jats:italic>\n                      strains were subjected to phylogrouping, as well as detection of 13 virulence genes and 45 resistance genes, using multiplex PCR. Antimicrobial susceptibility of the isolates to 22 antibiotics was determined by the disk diffusion method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Enteroaggregative\n                      <jats:italic>E. coli<\/jats:italic>\n                      accounted for 27.86% of isolates, and putative STEC\/EHEC accounted for 7.14%, with\n                      <jats:italic>astA<\/jats:italic>\n                      being the most prevalent (27.86%). Phenotypically, 94.29% were resistant to amoxicillin and 68.57% to sulfonamides, while 82.86% satisfied the MDR definition; genotypically,\n                      <jats:italic>tetA<\/jats:italic>\n                      (70.71%) and\n                      <jats:italic>floR<\/jats:italic>\n                      (57.14%) dominated the resistance repertoire. Phylogroups A and B1 predominated, but resistance and virulence profiles were dispersed across all lineages, indicating horizontal gene flow rather than clonal expansion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Tibetan pigs represent a substantial reservoir of MDR\n                      <jats:italic>E. coli<\/jats:italic>\n                      , strengthening the rationale for farm\u2010level antimicrobial stewardship and integrated surveillance that links animal, environmental and human compartments. The study is limited by its cross\u2010sectional design, focus on a single province and animal host and the absence of parallel human or environmental isolates, which prevent inference of causal zoonotic pathways and should be addressed in longitudinal One Health investigations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70899","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T12:23:54Z","timestamp":1775219034000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathogenicity and Antimicrobial\u2010Resistance Profiles of\n                    <i>Escherichia coli<\/i>\n                    Isolated From Faeces of Tibetan Pigs Reared Under Intensive Conditions"],"prefix":"10.1002","volume":"12","author":[{"given":"Runbo","family":"Luo","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Nanjing Agricultural University  Nanjing People's Republic of China"},{"name":"College of Animal Science Xizang Agricultural and Animal Husbandry University  Linzhi People's Republic of China"},{"name":"Key Laboratory for Prevention and Control of Hydatid Disease in Xizang (Co\u2010constructed by Ministry and Province) Ministry of Agriculture and Rural Affairs  Linzhi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-5341-576X","authenticated-orcid":false,"given":"Lihong","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Gansu Agricultural University  Lanzhou Gansu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Li","sequence":"additional","affiliation":[{"name":"College of Animal Science Xizang Agricultural and Animal Husbandry University  Linzhi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanan","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Animal Science Xizang Agricultural and Animal Husbandry University  Linzhi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Shang","sequence":"additional","affiliation":[{"name":"College of Animal Science Xizang Agricultural and Animal Husbandry University  Linzhi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suolang","family":"Sizhu","sequence":"additional","affiliation":[{"name":"College of Animal Science Xizang Agricultural and Animal Husbandry University  Linzhi People's Republic of China"},{"name":"Key Laboratory for Prevention and Control of Hydatid Disease in Xizang (Co\u2010constructed by Ministry and Province) Ministry of Agriculture and Rural Affairs  Linzhi People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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