{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T09:49:34Z","timestamp":1791798574585,"version":"4.3.4"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T00:00:00Z","timestamp":1773964800000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Cystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of\n                      <jats:italic>Echinococcus granulosus<\/jats:italic>\n                      and associated with productivity and economic losses in the livestock population across the world.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This cross\u2010sectional study spanned over a period of 1 year (2024) to determine the seroprevalence and associated risk factors of CE in large ruminants (cattle and buffaloes) hosted in different districts of Punjab, Pakistan, using an in\u2010house developed ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Overall, 430 serum samples from 197 buffaloes and 233 cattle were collected for detection of anti\u2010\n                      <jats:italic>E. granulosus<\/jats:italic>\n                      antibodies using an in\u2010house ELISA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The highest seropositivity rate (68.24%) was in cattle, whereas buffaloes had 19.28%. The geographical variation revealed that the lowest seropositivity rate (9.52%) was in Haroonabad, whereas Bahawalpur had the highest seropositivity rate (96.29%). Sex\u2010wise, females showed a seroprevalence of 46.13%, whereas males had a seroprevalence of 37.50%. Age\u2010wise trends showed that seropositivity increased up to 10\u201312 years of age, while decreasing in older animals. Breed\u2010wise, the highest seropositivity rate of 96.29% was observed in the Cholistani cattle, and the lowest rate of 25.25% was shown by Nili\u2010Ravi buffaloes. The LASSO model revealed a high prediction accuracy of 76.98% and an area under the curve (0.73), suggesting geographical and host\u2010related impacts on the epidemiology of CE. However, our findings indicate widespread seropositivity, the study does not evaluate production losses or economic impact, and our conclusion refers to epidemiological patterns.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>There is a prompt need for targeted strategies under the One Health concept for region\u2010specific surveillance, improving animal husbandry practices and controlling definitive hosts, particularly in high\u2010risk areas of Pakistan.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70901","type":"journal-article","created":{"date-parts":[[2026,3,20]],"date-time":"2026-03-20T12:20:28Z","timestamp":1774009228000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Seroprevalence and Risk Factors of Cystic Echinococcosis in Cattle and Buffaloes: Insights From an In\u2010House ELISA"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8800-2953","authenticated-orcid":false,"given":"Mughees Aizaz","family":"Alvi","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences Lanzhou China"},{"name":"National Para\u2010Reference Laboratory for Animal Echinococcosis, Gansu Province Research Center for Basic Disciplines of Pathogen Biology Key Laboratory of Veterinary Parasitology of Gansu Province, Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Ruminant Disease Prevention and Control (West), Ministry of Agricultural and Rural Affairs  Lanzhou China"},{"name":"Department of Clinical Medicine and Surgery University of Agriculture  Faisalabad 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