{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:47:13Z","timestamp":1791906433455,"version":"4.3.4"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T00:00:00Z","timestamp":1774828800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Subclinical mastitis is the predominant form of mammary inflammation in dairy buffaloes and remains a diagnostic challenge under field conditions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study assessed milk C\u2010reactive protein (CRP) concentrations in relation to somatic cell count (SCC) and microbiological findings in Anatolian and Italian Mediterranean buffaloes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Milk samples were collected from 160 individual mammary lobes and classified as subclinical mastitis\u2010positive or healthy based on combined cytological and bacteriological criteria. SCC was determined using an automated cell counter, microbiological examination was performed by conventional culture and identification methods, and milk CRP concentrations were quantified by a sandwich ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      SCC was significantly higher in subclinical mastitis\u2010affected mammary lobes than in healthy controls in both breeds (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). Mean SCC values were 1616.05 \u00b1 167.04 \u00d710\n                      <jats:sup>3<\/jats:sup>\n                      cells\/mL and 1245.98 \u00b1 82.90 \u00d710\n                      <jats:sup>3<\/jats:sup>\n                      cells\/mL in subclinical mastitis samples from Anatolian Mediterranean and Italian Mediterranean buffaloes, respectively, whereas corresponding control values were 285.48 \u00b1 19.96 \u00d710\n                      <jats:sup>3<\/jats:sup>\n                      cells\/mL and 254.45 \u00b1 21.44 \u00d710\n                      <jats:sup>3<\/jats:sup>\n                      cells\/mL (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). Bacterial growth was detected only in samples classified as subclinical mastitis, with\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      identified as the most frequent isolate. Mean milk CRP concentrations were significantly higher in subclinical mastitis samples compared with controls, with values of 196.26 \u00b1 3.44\u00a0ng\/mL and 205.45 \u00b1 5.89\u00a0ng\/mL in subclinical mastitis samples from Anatolian Mediterranean and Italian Mediterranean buffaloes, respectively, versus 162.16 \u00b1 4.95\u00a0ng\/mL and 158.22 \u00b1 3.32\u00a0ng\/mL in control samples (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001). Breed did not influence milk CRP concentrations within the same mastitis status.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results indicate that milk CRP reflects mammary inflammatory activity and provides information complementary to SCC in the evaluation of subclinical mastitis in dairy buffaloes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70924","type":"journal-article","created":{"date-parts":[[2026,3,30]],"date-time":"2026-03-30T11:49:24Z","timestamp":1774871364000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Association of Milk C\u2010Reactive Protein With Subclinical Mastitis in Dairy Buffaloes"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9080-7056","authenticated-orcid":false,"given":"Ahmet","family":"Sabuncu","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2352-876X","authenticated-orcid":false,"given":"Sinem \u00d6zlem","family":"Enginler","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9996-3290","authenticated-orcid":false,"given":"Gamze","family":"Evkuran Dal","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9013-930X","authenticated-orcid":false,"given":"Atila","family":"Ate\u015f","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9122-0284","authenticated-orcid":false,"given":"Baran","family":"\u00c7elik","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6502-0780","authenticated-orcid":false,"given":"Serkan","family":"Ikiz","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2827-4471","authenticated-orcid":false,"given":"\u00d6m\u00fcr","family":"Ko\u00e7ak","sequence":"additional","affiliation":[{"name":"Department of Animal Breeding and Husbandry, Faculty of Veterinary Medicine \u0130stanbul University\u2010Cerrahpa\u015fa  \u0130stanbul T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2107-1874","authenticated-orcid":false,"given":"Asl\u0131han","family":"Baykal U\u011fur","sequence":"additional","affiliation":[{"name":"Department of Obstetrics 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