{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:46:51Z","timestamp":1791906411148,"version":"4.3.4"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Investigation on\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      transmission between pets and their close human contacts remains limited.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Here, we assessed\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      prevalence in companion animals and their contacts.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Faecal samples from dogs and cats (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0352) and associated humans (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0108) were screened by nested PCR targeting the SSUrRNA gene, and the positive specimens were subtyped by sequencing the 60\u2010kDa glycoprotein gene (\n                      <jats:italic>gp60<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The infection rates of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. were 11.1% in dogs and 10.4% in cats, and dogs with diarrhoea were more likely to be infected by\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. (47.8%; odds ratio [OR]\u00a0=\u00a014.3,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.001). Among human specimens, only a 62\u2010year\u2010old owner who had long\u2010term contact with rescued stray cats tested positive for\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp.\n                      <jats:italic>Cryptosporidium canis<\/jats:italic>\n                      was detected in dogs, and\n                      <jats:italic>Cryptosporidium felis<\/jats:italic>\n                      was detected in cats and the owner. The\n                      <jats:italic>C. canis<\/jats:italic>\n                      isolates comprised both zoonotic (XXa and XXb) and dog\u2010adapted (XXd and XXe) subtypes, whereas all\n                      <jats:italic>C. felis<\/jats:italic>\n                      isolates clustered within zoonotic subtype family XIXa. Notably, the\n                      <jats:italic>C. felis<\/jats:italic>\n                      isolates from the owner and the two cats were found to have an identical\n                      <jats:italic>gp60<\/jats:italic>\n                      sequence to a known human subtype XIXa\u201040. In addition, young dogs shed significantly more\n                      <jats:italic>C. canis<\/jats:italic>\n                      oocysts per gram of faeces (4000) than adult dogs (\u223c2000).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our results showed that transmission of pet cryptosporidiosis to adults was minimal, but spillover to immunocompromised elderly individuals could pose a public health concern.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70961","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T11:25:14Z","timestamp":1777116314000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Zoonotic Transmission Assessment of\n                    <i>Cryptosporidium<\/i>\n                    spp. in Close Human\u2013Pet Environments in Yunnan Province, China"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-5991-9331","authenticated-orcid":false,"given":"Rongchang","family":"Cao","sequence":"first","affiliation":[{"name":"School of Animal Medicine Yunnan Agricultural University  Kunming Yunnan China"},{"name":"Dali Vocational and Technical College of Agriculture and Forestry  Dali Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3259-1620","authenticated-orcid":false,"given":"Dongxu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Animal Medicine Yunnan Agricultural University  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8725-0718","authenticated-orcid":false,"given":"Yin","family":"Zhou","sequence":"additional","affiliation":[{"name":"School of Animal Medicine Yunnan Agricultural University  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3957-1158","authenticated-orcid":false,"given":"Chunyan","family":"Li","sequence":"additional","affiliation":[{"name":"Ruichong Animal Hospital  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-9659-7822","authenticated-orcid":false,"given":"Fanfan","family":"Shu","sequence":"additional","affiliation":[{"name":"School of Animal Medicine Yunnan Agricultural University  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9449-8668","authenticated-orcid":false,"given":"Jiayu","family":"Li","sequence":"additional","affiliation":[{"name":"School of Animal Medicine Yunnan Agricultural University  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,25]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/20477724.2023.2290379"},{"issue":"1","key":"e_1_2_12_3_1","first-page":"60","article-title":"BioEdit: An Important Software for Molecular Biology","volume":"2","author":"Alzohairy A. 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