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It has zoonotic potential and poses a threat to human health, as infection can occur through the consumption of raw or undercooked fish.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      The present study was designed to characterize\n                      <jats:italic>Eustrongylides<\/jats:italic>\n                      sp. isolated from\n                      <jats:italic>Channa punctata<\/jats:italic>\n                      using the\n                      <jats:italic>Internal Transcribed Spacer 2<\/jats:italic>\n                      (\n                      <jats:italic>ITS2<\/jats:italic>\n                      ) regions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In this study, a total of 137 medium\u2010sized\n                      <jats:italic>C. punctata<\/jats:italic>\n                      (17.5\u00a0\u00b1\u00a02.5\u00a0cm) were collected from different locations of Jamalpur and Netrokona district, Bangladesh and isolated 44 nematodes. DNA was extracted from the parasites, amplified in the\n                      <jats:italic>ITS2<\/jats:italic>\n                      region and sequenced.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All the nematodes were microscopically identified as belonging to the genus\n                      <jats:italic>Eustrongylides<\/jats:italic>\n                      based on morphological characteristics, including the presence of inner and outer circle labial papillae at the anterior end and the esophago\u2010intestinal junction. Initially, the sequences were confirmed up to the lowest taxonomic level possible using Basic Local Alignment Search Tool (BLAST). For phylogenetic analysis, reference sequences of\n                      <jats:italic>Eustrongylides<\/jats:italic>\n                      sp. were aligned, and two single nucleotide polymorphisms (SNPs) were detected. The average sequence identity percentage (%) within the studied sequences ranged from 99.7% to 99.8%. A maximum\u2010likelihood (ML) tree was constructed which showed that the studied sequences clustered within the\n                      <jats:italic>Eustrongylides<\/jats:italic>\n                      sp. lineage and grouped with reference sequences, supported by moderate bootstrap values (82%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Close genetic similarity was observed when compared with reference sequences of\n                      <jats:italic>Eustrongylides<\/jats:italic>\n                      sp. Further studies on the investigation of genetic pattern of\n                      <jats:italic>Eustrongylides<\/jats:italic>\n                      sp. throughout the country are essential to control this parasite.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70965","type":"journal-article","created":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T15:13:36Z","timestamp":1777475616000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization of\n                    <i>Eustrongylides<\/i>\n                    sp. Infecting\n                    <i>Channa punctata<\/i>\n                    in Bangladesh"],"prefix":"10.1002","volume":"12","author":[{"given":"Salman Shahriar","family":"Nibir","sequence":"first","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5462-7982","authenticated-orcid":false,"given":"Anita Rani","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3586-0938","authenticated-orcid":false,"given":"Tanvir","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julfat Tasnim","family":"Suchona","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyam","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Savayan Sadad","family":"Bushra","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jubaida Parveen","family":"Juthy","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandhya","family":"Paul","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarin Tasnim","family":"Tanwi","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamanna","family":"Tabassum","sequence":"additional","affiliation":[{"name":"Department of Aquaculture Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. 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