{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:19:39Z","timestamp":1787919579231,"version":"build-2784847793"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine & Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Background<\/jats:title>\n Gonadotropins are glycoprotein hormones that are often secreted from the gonadotrope cells of the pituitary gland. These hormones play an essential role in growth, reproduction and sexual function.<\/jats:p>\n <\/jats:sec>\n \n Aims<\/jats:title>\n \n Therefore, this research was conducted to identify genetic polymorphisms of the Gonadotropin\u2010Releasing Hormone Receptor (\n GnRHR<\/jats:italic>\n ) gene on Isfahan native hen traits and their relationship with production traits.\n <\/jats:p>\n <\/jats:sec>\n \n Materials & methods<\/jats:title>\n For this purpose, blood was taken from 100 Isfahan native hens that were randomly selected from the Isfahan native hen centre. DNA was extracted by the phenol\u2010chloroform method. Then, using the designed primers, the target fragment was amplified and directly sequenced. The sequences were blasted with the reference gene in NCBI. After analysing the sequences, the relationship between genotypes and traits was investigated.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The results showed that five single\u2010nucleotide polymorphisms in gene\n GNRHR<\/jats:italic>\n were observed. These polymorphisms were located in nucleotides numbers 117, 123, 157, 453 and 550 of the amplified fragments and caused the change of nucleotide T to C, C to T, C to T, C to T and A to G, respectively. According to the results, the\n GnRHR<\/jats:italic>\n gene has an acceptable effect on the productive traits of Isfahan native hens. Of course, its effect cannot be considered important for the entire production traits, but in the sexual maturity sections, the number of eggs plays an important role and has a significant effect on the mentioned production traits.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n These results show that the observed nucleotide changes if confirmed in other relevant studies can be used in the trait selection programme with the help of markers.<\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/vms3.70969","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T13:07:42Z","timestamp":1779973662000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Polymorphism of the Gonadotropin\u2010Releasing Hormone Receptor Gene and Its Association With Production Traits in Isfahani Native Hens"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5517-4524","authenticated-orcid":false,"given":"Farshid","family":"Kheiri","sequence":"first","affiliation":[{"name":"Department of Animal Sciences ShK.C., Islamic Azad University Shahrekord Iran"},{"name":"Biotechnology Research Center ShK.C., Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1215-419X","authenticated-orcid":false,"given":"Mostafa","family":"Faghani","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences ShK.C., Islamic Azad University Shahrekord Iran"},{"name":"Biotechnology Research Center ShK.C., Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Mohammadi","family":"Berenjegani","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences ShK.C., Islamic Azad University Shahrekord Iran"},{"name":"Department of Animal Sciences College of Agriculture Isfahan University of Technology Isfahan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somayeh Tirbakhsh","family":"Gouran","sequence":"additional","affiliation":[{"name":"Department of Biology ShK.C., Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matin Rahimi","family":"Domakani","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences ShK.C., Islamic Azad University Shahrekord Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/epsj.2023.305162"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10050384"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2527\/msasas2016-325"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.027060"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/00071668.2020.1758299"},{"issue":"2","key":"e_1_2_10_7_1","first-page":"193","article-title":"Molecular Detection of Some Growth\u2010Hormone Genes in Chickens","volume":"100","author":"El\u2010Sayed M. A.","year":"2022","journal-title":"Egyptian Journal of Agricultural Research"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.1388"},{"issue":"6","key":"e_1_2_10_9_1","first-page":"518","article-title":"Induced Spawning of Schizothorax zarudnyi (Cyprinidae) by Using Synthetic Hormones (Ovaprim and HCG)","volume":"3","author":"Gharaei A.","year":"2011","journal-title":"World Journal of Fish and Marine Sciences"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.1296"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0739-7240(88)90017-3"},{"key":"e_1_2_10_12_1","first-page":"7","article-title":"Growth Parameters Evaluation and Identification of Growth Hormone Receptor Gene Polymorphisms in Various Strains of Rainbow Trout Oncorhynchus mykiss","volume":"7","author":"Gorji A.","year":"2016","journal-title":"Journal of Aquaculture Research & Development"},{"issue":"4","key":"e_1_2_10_13_1","first-page":"408","article-title":"Hypothalamic\u2010Pituitary\u2010Go Nad al (HPG) Axis and Its Functional Regulation in Birds","volume":"19","author":"Kataki B.","year":"2022","journal-title":"BIOINFOLET\u2010A Quarterly Journal of Life Sciences"},{"issue":"8","key":"e_1_2_10_14_1","first-page":"1202","article-title":"Effect of Use Different Levels of Caraway (Carum carvi L.) Powder On Performance, Some Blood Parameters and Intestinal Morphology On Broiler Chickens","volume":"19","author":"Khajeali Y.","year":"2012","journal-title":"World Applied Sciences Journal"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.13412"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.4236\/ojas.2016.61001"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygcen.2004.09.015"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.48165\/ijvsbt.19.2.18"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.48165\/ijvsbt.18.5.21"},{"issue":"2","key":"e_1_2_10_20_1","first-page":"34","article-title":"Estimation of Genetic Parameters for Productive and Reproductive Traits in Esfahan Native Chickens","volume":"1","author":"Yousefi Zonuz A.","year":"2013","journal-title":"Journal of Livestock Science and Technologies"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/ps\/84.8.1209"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70969","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.70969","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.70969","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:15:08Z","timestamp":1787919308000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.70969"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,28]]},"references-count":20,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1002\/vms3.70969"],"URL":"https:\/\/doi.org\/10.1002\/vms3.70969","archive":["Portico"],"relation":{},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,28]]},"assertion":[{"value":"2024-02-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-09","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-28","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70969"}}