{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T15:46:44Z","timestamp":1791906404890,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T00:00:00Z","timestamp":1777334400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Avian metapneumovirus (aMPV) and infectious laryngotracheitis virus (ILTV) are globally important respiratory pathogens in poultry. However, their serological prevalence has not been established in Ghana. The study therefore sought to serologically detect aMPV and ILTV in commercial poultry in the Ashanti Region of Ghana.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This cross\u2010sectional serological study was conducted in the Ashanti Region, one of the main poultry\u2010producing zones in Ghana. Antibodies to aMPV and ILTV were determined in serum samples collected from 300 clinically healthy chickens from thirteen large commercial farms, using commercially available indirect ELISA kits (IDvet, France).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overall seroprevalence was 49.0% (147\/300; 95% CI: 43.3%\u201354.7%) for aMPV and 7.0% (21\/300; 95% CI: 4.4%\u201310.6%) for ILTV. Farm\u2010level seroprevalence of aMPV ranged from 0% to 100%, while ILTV ranged from 0% to 40%. Co\u2010exposure with both viruses was detected in 5.0% (15\/300) of birds. Fisher's exact test revealed a statistically significant positive association between aMPV and ILTV seropositivity (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.041; odds ratio = 2.78; 95% CI: 0.98\u20138.99).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The study provides the first serological findings of exposure of aMPV and ILTV in commercial poultry flocks in Ghana. The high levels of aMPV together with the ILTV detection are a true indication that the need to improve respiratory disease surveillance, diagnostic capacity and analyse potential vaccination options of the Ghanaian poultry industry is urgent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.70971","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T07:29:14Z","timestamp":1777361354000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Serological Evidence of Avian Metapneumovirus and Infectious Laryngotracheitis Virus in Commercial Poultry in the Ashanti Region of Ghana"],"prefix":"10.1002","volume":"12","author":[{"given":"Patrick Mensah","family":"Amponsah","sequence":"first","affiliation":[{"name":"Department of Theoretical and Applied Biology Kwame Nkrumah University of Science and Technology (KNUST)  Kumasi Ghana"},{"name":"Kumasi Veterinary Laboratory  Amakom Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5014-6153","authenticated-orcid":false,"given":"Kwadwo","family":"Boampong","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology Kwame Nkrumah University of Science and Technology (KNUST)  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7691-914X","authenticated-orcid":false,"given":"Augustina Angelina","family":"Sylverken","sequence":"additional","affiliation":[{"name":"Department of Theoretical and Applied Biology Kwame Nkrumah University of Science and Technology (KNUST)  Kumasi Ghana"},{"name":"Kumasi Centre for Collaborative Research in Tropical Medicine Kwame Nkrumah University of Science and Technology (KNUST)  Kumasi Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani14223227"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40011-023-01535-7"},{"key":"e_1_2_13_4_1","first-page":"15","article-title":"Seroprevalence and Associated Risk Factors of Co\u2010Infection With Avian Influenza and Newcastle Disease in Guinea Fowls From the Upper East Region of Ghana","author":"Albert T. 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