{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:13:27Z","timestamp":1785338007632,"version":"3.55.0"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T00:00:00Z","timestamp":1642464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FSSA_1_2019_1_160057"],"award-info":[{"award-number":["FSSA_1_2019_1_160057"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine & Sci"],"published-print":{"date-parts":[[2022,3]]},"abstract":"Abstract<\/jats:title>\n \n Listeriosis is one of the most common nervous diseases in ruminants, and is caused almost exclusively by the Gram\u2010positive bacterium,\n Listeria monocytogenes<\/jats:italic>\n . However, there are few reports of listeriosis associated with\n L. innocua<\/jats:italic>\n , which is genetically closely related to\n L. monocytogenes<\/jats:italic>\n , but considered non\u2010pathogenic. In this work, we report two cases of suppurative meningoencephalitis in apparently previously healthy ruminants from different farms, in which two strains of\n L. innocua<\/jats:italic>\n were recovered. The whole genomes from both isolates were sequenced, allowing phylogenetic analyses to be performed, which indicated that the two strains were very closely related. Virulence determinants were searched, especially genes coding for the main\n L. monocytogenes<\/jats:italic>\n virulence factors which have been previously described in\n L. innocua<\/jats:italic>\n . Surprisingly, the two isolates do not possess such virulence determinants. Instead, both strains carried a set of genes that encode for other virulence factors of the genus\n Listeria<\/jats:italic>\n detected\u00a0 using the Virulence Factor Database (VFDB):\n iap<\/jats:italic>\n (division and invasion of host cells),\n lpeA<\/jats:italic>\n (entry into non\u2010professional phagocytes cells),\n fbpA<\/jats:italic>\n (multifunctional virulence factor, including adherence to host cells)\n , lspA<\/jats:italic>\n (surface protein anchoring),\n lap<\/jats:italic>\n (adhesion to enterocytes and trans epithelial translocation)\n , pdgA<\/jats:italic>\n (resistance to lysozyme),\n oatA<\/jats:italic>\n (resistance to different antimicrobial compounds and also required for growth inside macrophages)\n , lplA1<\/jats:italic>\n (use of host\u2010metabolites for in vivo growth),\n gtcA<\/jats:italic>\n (catalyses teichoic acid of bacterial wall),\n prsA2<\/jats:italic>\n (cell invasion, vacuole lysis and intracellular growth),\n clpC, clpE<\/jats:italic>\n and\n clpP<\/jats:italic>\n (survival under several stress conditions). These genes among others detected, could be involved in the ability of\n L. innocua<\/jats:italic>\n to produce damage in animal and human hosts. These results highlight the multifactorial profile of\n Listeria<\/jats:italic>\n pathogenesis and the need for comprehensive scientific research that address microbiological, environmental and veterinary aspects of listeriosis.\n <\/jats:p>","DOI":"10.1002\/vms3.710","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T04:37:53Z","timestamp":1642480673000},"page":"735-740","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Listeria innocua<\/i>\n isolated from diseased ruminants harbour minor virulence genes of\n L. monocytogenes<\/i>"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7902-8676","authenticated-orcid":false,"given":"Carolina","family":"Matto","sequence":"first","affiliation":[{"name":"Laboratorio Regional Noroeste DILAVE \u2018Miguel C. Rubino\u2019 DGSG\u2010MGAP Paysand\u00fa Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"D'Alessandro","sequence":"additional","affiliation":[{"name":"Departamento de Desarrollo Biotecnol\u00f3gico Instituto de Higiene, Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda In\u00e9s","family":"Mota","sequence":"additional","affiliation":[{"name":"Departamento de Bacteriolog\u00eda y Virolog\u00eda Instituto de Higiene, Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Braga","sequence":"additional","affiliation":[{"name":"Departamento de Bacteriolog\u00eda y Virolog\u00eda Instituto de Higiene, Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Buschiazzo","sequence":"additional","affiliation":[{"name":"Laboratorio de Microbiolog\u00eda Molecular y Estructural Institut Pasteur de Montevideo Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgardo","family":"Gianneechini","sequence":"additional","affiliation":[{"name":"Laboratorio Regional Noroeste DILAVE \u2018Miguel C. Rubino\u2019 DGSG\u2010MGAP Paysand\u00fa Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Varela","sequence":"additional","affiliation":[{"name":"Departamento de Bacteriolog\u00eda y Virolog\u00eda Instituto de Higiene, Facultad de Medicina Universidad de la Rep\u00fablica Montevideo Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Rivero","sequence":"additional","affiliation":[{"name":"Laboratorio Regional Noroeste DILAVE \u2018Miguel C. Rubino\u2019 DGSG\u2010MGAP Paysand\u00fa Uruguay"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,1,18]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0928-8244(02)00448-0"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00516-10"},{"key":"e_1_2_10_5_1","article-title":"Listeria cossartiae sp.nov., Listeria farberi sp. nov., Listeria immoviles sp.nov., Listeria portnoyi sp. nov., and Listeria rustica sp. nov., isolated from agricultural water and natural environments","volume":"71","author":"Carlin C.R.","year":"2021","journal-title":"International Journal of Systematic and Evolutionary Microbiology"},{"key":"e_1_2_10_6_1","article-title":"Atypical Listeria innocua strains possess an intact LIPI\u20103","volume":"14","author":"Clayton E.M.","year":"2013","journal-title":"BMC Microbiology"},{"key":"e_1_2_10_7_1","article-title":"First case of Listeria innocua meningitis in a patient on steroids and eternecept","author":"Favaro M.","year":"2014","journal-title":"Journal of Medical Microbiology Case Reports"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.05307-11"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_2_10_10_1","unstructured":"Hitchins A.D. Jinneman K. &ChenY.(2020). Detection ofListeria monocytogenesin foods and environmental samples and enumeration ofListeria monocytogenesin foods. Bacteriological Analytical Manual.United States Food and Drug Administration. 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