{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:19:10Z","timestamp":1787919550846,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T00:00:00Z","timestamp":1781222400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>In this study, it was aimed to compare the effects of embryo transfer performed via laparotomy and laparoscopy on thiol\u2013disulphide homeostasis and some oxidative stress markers in Tuj ewes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In the study, 10 Tuj ewes were randomly divided into two groups (laparotomy,\n                      <jats:italic>n<\/jats:italic>\n                      = 5; laparoscopy,\n                      <jats:italic>n<\/jats:italic>\n                      = 5). A progesterone\u2010based synchronization protocol was applied to all animals. Blood samples were collected immediately before surgery, immediately after surgery, and at 24 and 48 h postoperatively. Serum levels of glutathione (GSH), malondialdehyde (MDA), total thiol (TT), native thiol (NT), disulphide (Ds) and thiol\u2013disulphide ratios were determined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Group, time, and group \u00d7 time interactions were found to be statistically significant for GSH, MDA, TT, and NT parameters (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). In the laparotomy group, GSH, TT, and NT levels markedly decreased during the postoperative period (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05), whereas MDA levels showed a significant increase, particularly at 48 h (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). In the laparoscopy group, changes in oxidative stress markers were found to be more limited. Although no significant differences were observed between the groups in terms of disulphide levels and thiol\u2013disulphide ratios (\n                      <jats:italic>p<\/jats:italic>\n                      &gt; 0.05), time\u2010dependent changes in these parameters were statistically significant (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). In the laparotomy group, significant negative correlations were detected between MDA and antioxidant parameters (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05), while strong positive correlations were observed among thiol parameters (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>As a result, it was concluded that the surgical approach used in embryo transfer affects the systemic oxidative stress response and that thiol\u2013disulphide homeostasis can be considered a sensitive biomarker for revealing these differences. It was determined that the laparoscopic embryo transfer method disrupts redox balance to a lesser extent and better preserves the antioxidant defence system compared with laparotomy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71023","type":"journal-article","created":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T08:22:51Z","timestamp":1781252571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Effects of Laparotomy and Laparoscopy\u2010Assisted Embryo Transfer on Oxidative Stress and Thiol\u2013Disulphide Homeostasis in Tuj Ewes"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3803-069X","authenticated-orcid":false,"given":"Murat Can","family":"Demir","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine Kafkas University  Kars 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