{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:19:22Z","timestamp":1787919562411,"version":"build-2784847793"},"reference-count":24,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Inclusion body hepatitis (IBH), caused by fowl adenoviruses (FAdVs), is an emerging disease of commercial broilers associated with significant economic losses. In Malaysia, molecular epidemiological data on circulating FAdV serotypes have largely been restricted to Peninsular regions, with limited information available from East Malaysia (Sabah), despite its rapidly expanding poultry industry and distinct production systems. This regional knowledge gap limits comprehensive understanding of FAdV transmission dynamics at the national level.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to detect FAdV infection in broiler flocks with IBH\u2010compatible lesions in Sabah, Malaysia, and to characterize circulating serotypes and their phylogenetic relationships.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Thirty pooled tissue samples (liver and gizzard) and 60 serum samples were collected from three broiler farms (YAN, KON, and NIS). FAdV detection was performed using polymerase chain reaction (PCR) targeting the\n                      <jats:italic>hexon<\/jats:italic>\n                      gene. Serological responses were assessed using enzyme\u2010linked immunosorbent assay (ELISA). Positive PCR products were sequenced, and phylogenetic analysis was conducted to determine serotype distribution and genetic relatedness.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      FAdV DNA was detected in 33.3% (10\/30; 95% CI: 17.3\u201352.8) of pooled tissue samples, with detection rates of 70% (95% CI: 44.4\u201397.5), 20% (95% CI: 2.5\u201355.6) and 10% (95% CI: 0.3\u201344.5) in the YAN, KON and NIS farms, respectively. Although descriptive differences were observed among farms, these variations were not statistically significant (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &gt;\u00a00.05). All positive samples yielded the expected 897\u00a0bp\n                      <jats:italic>hexon<\/jats:italic>\n                      gene amplicon. ELISA revealed high seropositivity in unvaccinated flocks, with the highest mean antibody titre observed in the YAN farm (24,716.5\u00a0\u00b1\u00a04516.1). Molecular characterization identified FAdV\u20108b (species E) and FAdV\u201011 (species D), indicating co\u2010circulation. Phylogenetic analysis showed close relatedness to strains from Korea, Belgium, China and Australia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The co\u2010circulation of FAdV\u20108b and FAdV\u201011 in Sabah broiler farms underscores the need for continuous molecular surveillance, enhanced biosecurity and breeder\u2010level vaccination strategies using locally circulating FAdV strains to control IBH in Malaysia.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71033","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T10:27:25Z","timestamp":1782210445000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integrating Serological and Molecular Data to Characterize Fowl Adenovirus Associated With Inclusion Body Hepatitis in Broiler Chickens From Malaysia"],"prefix":"10.1002","volume":"12","author":[{"given":"Md. Safiul Alam","family":"Bhuiyan","sequence":"first","affiliation":[{"name":"Faculty of Sustainable Agriculture Universiti Malaysia Sabah  Sandakan Sabah Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Babu Kanti","family":"Nath","sequence":"additional","affiliation":[{"name":"Biosecurity, Gulbali Institute School of Agricultural Environmental and Veterinary Sciences Charles Sturt University  Wagga Wagga New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6819-8517","authenticated-orcid":false,"given":"Lum Mok","family":"Sam","sequence":"additional","affiliation":[{"name":"Faculty of Sustainable Agriculture Universiti Malaysia Sabah  Sandakan Sabah Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnan Nair","family":"Balakrishnan","sequence":"additional","affiliation":[{"name":"Biotechnology Research Institute Universiti Malaysia Sabah  Kota Kinabalu Sabah Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suman Das","family":"Gupta","sequence":"additional","affiliation":[{"name":"Biosecurity, Gulbali Institute School of Agricultural Environmental and Veterinary Sciences Charles Sturt University  Wagga Wagga New South Wales Australia"},{"name":"School of Agricultural Environmental and Veterinary Sciences Faculty of Science and Health Charles Sturt University  Wagga Wagga New South Wales Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2685-8377","authenticated-orcid":false,"given":"Subir","family":"Sarker","sequence":"additional","affiliation":[{"name":"Biomedical Sciences &amp; Molecular Biology College of Medicine and Dentistry James Cook University  Townsville Queensland Australia"},{"name":"Department of Microbiology Anatomy, Physiology and Pharmacology School of Agriculture Biomedicine and Environment La Trobe University  Melbourne Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2025.107482"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.35118\/apjmbb.2024.032.4(Special).08"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-024-04081-0"},{"key":"e_1_2_13_5_1","first-page":"23","article-title":"Inclusion Body Hepatitis in a Flock of Commercial Broiler Chickens","volume":"17","author":"Bejo M. H.","year":"2005","journal-title":"Journal of Veterinary Malaysia"},{"key":"e_1_2_13_6_1","first-page":"125","volume-title":"Taxonomy: Classification and Nomenclature of Viruses. 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