{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:16:38Z","timestamp":1787922998166,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and aim<\/jats:title>\n                    <jats:p>Orf virus (ORFV) is the etiologic agent of infectious ecthyma, a common worldwide disease that occasionally causes zoonotic infections. In this study, we aimed to detect and molecularly characterise circulating ORFV strains in goats from different provinces in eastern T\u00fcrkiye.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>\n                      Skin lesion samples collected during ORFV outbreaks between 2022 and 2024 were analysed. Following DNA extraction, positive samples identified by real\u2010time PCR were analysed using a multi\u2010locus phylogenetic approach targeting the structural\n                      <jats:italic>F1L<\/jats:italic>\n                      , the major envelope protein\n                      <jats:italic>B2L<\/jats:italic>\n                      , and the virulence\u2010related\n                      <jats:italic>VIR<\/jats:italic>\n                      gene regions. Sequencing was performed using the Sanger method, followed by phylogenetic analysis using the Maximum Likelihood method in MEGA X.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Phylogenetic analysis revealed high genetic similarity (95.63% to 99.48%) between the Turkish strains and global isolates from countries such as China, India, Iran, and Malaysia. Notably, while most isolates shared 100% similarity, the isolate from one specific province (Tunceli) exhibited significant nucleotide substitutions and a separate clustering pattern, particularly in the\n                      <jats:italic>VIR<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The results demonstrate that ORFV strains circulating in T\u00fcrkiye display close phylogenetic clustering with Asian strains. The study highlights that multi\u2010locus analyses, especially utilising the highly variable\n                      <jats:italic>VIR<\/jats:italic>\n                      gene, are essential for identifying regional genetic heterogeneity and monitoring microbial evolution. These findings provide a pilot reference for future epidemiological surveillance and vaccine development strategies in the region.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71044","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T12:52:06Z","timestamp":1781873526000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterisation of Orf Virus in Goats From Eastern T\u00fcrkiye"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9831-7193","authenticated-orcid":false,"given":"Remziye","family":"\u00d6zbek","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Virology Sivas Cumhuriyet University  Sivas T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehmet","family":"Ilg\u0131n","sequence":"additional","affiliation":[{"name":"Elaz\u0131\u011f Veterinary Control Institute  Elaz\u0131\u011f T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kezban","family":"Can\u2010\u015eahna","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Virology Dokuz Eyl\u00fcl University  \u0130zmir T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378\u20101135(95)00055\u2010f"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743\u2010422X\u20109\u201024"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2011.09.005"},{"issue":"7","key":"e_1_2_11_5_1","first-page":"684","article-title":"Orf Virus Infection in Humans: A Review With a Focus on Advances in Diagnosis and Treatment","volume":"16","author":"Caravaglio J. 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