{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:16:34Z","timestamp":1787922994980,"version":"build-2784847793"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Encephalitozoon cuniculi<\/jats:italic>\n                      is an obligate intracellular microsporidian parasite that commonly infects rabbits and exhibits zoonotic potential by occasionally infecting humans. This study investigated the prevalence and genotypic distribution of\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      in pet rabbits in central Thailand.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      In total, 101 voided urine samples from owned pet rabbits were screened for\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      using polymerase chain reaction\u2013based technique targeting the internal transcribed spacer (ITS) region of ribosomal DNA. Fifteen positive samples were further analysed using multi\u2010locus genotyping (ITS region,\n                      <jats:italic>PTP<\/jats:italic>\n                      and\n                      <jats:italic>SWP1<\/jats:italic>\n                      ) and phylogenetic relationship analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In total, 21 (20.79%) pet rabbits tested positive for\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      . Although\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      prevalence was not associated with breed (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.508), sex (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a01.000) or age (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.413), it was significantly higher in clinically affected rabbits (46.43%; 95% confidence interval [CI]: 27.5%\u201366.1%) than in clinically healthy rabbits (4.88%; 95% CI: 0.6%\u201316.2%). Multi\u2010locus genotyping demonstrated the co\u2010circulation of Genotypes I and III within the population. Phylogenetic analysis also revealed a close genetic relationship between rabbit\u2010derived strains identified in the study and genotypes previously reported in humans.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study demonstrated the presence of\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      DNA in urine samples from both clinically affected and apparently healthy pet rabbits, with multi\u2010locus genotyping revealing the presence of\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      Genotypes I and III. To the best of our knowledge, this is the first report describing molecular detection and genotypic characterization of\n                      <jats:italic>E. cuniculi<\/jats:italic>\n                      in pet rabbits in Thailand.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71048","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T12:33:26Z","timestamp":1782995606000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Detection and Genotypic Analysis of\n                    <i>Encephalitozoon cuniculi<\/i>\n                    in Pet Rabbits in Central Thailand"],"prefix":"10.1002","volume":"12","author":[{"given":"Benjaporn","family":"Bhusri","sequence":"first","affiliation":[{"name":"Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals Faculty of Veterinary Science Mahidol University, Nakhon 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