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No prior molecular data existed on giardiasis in this host population.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This cross\u2010sectional study aimed to determine the prevalence and molecular diversity of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      spp. and\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      in equines across multiple regions of Algeria, and to characterise species, subtypes and assemblages present.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Between December 2021 and December 2022, 197 faecal samples were collected from 104 horses, 89 donkeys, 2 ponies, 1 zebra and 1 mule from 46 private farms, 2 equestrian centres and 1 zoological park in eastern and central Algeria. Detection used microscopy and PCR.\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      species and subtypes were identified by sequence analysis of SSU rRNA and\n                      <jats:italic>gp60<\/jats:italic>\n                      genes.\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      was screened by probe\u2010based qPCR with genotyping attempted at the\n                      <jats:italic>tpi<\/jats:italic>\n                      and\n                      <jats:italic>bg<\/jats:italic>\n                      loci.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The overall molecular prevalence of\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      was 34% (horses 31%, donkeys 38%), and\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      was 4.6% (horses 2.9%, donkeys 7%). Male equines had a significantly higher\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      infection rate than females; no other epidemiological variable was significant for either parasite. Ten\n                      <jats:italic>Cryptosporidium<\/jats:italic>\n                      isolates were genotyped as four species:\n                      <jats:italic>C. parvum<\/jats:italic>\n                      (subtype IIaA20G1R1),\n                      <jats:italic>C. equi<\/jats:italic>\n                      (VIaA15G3, VIaA11G2),\n                      <jats:italic>C. hominis<\/jats:italic>\n                      (IkA15G1) and\n                      <jats:italic>C. muris<\/jats:italic>\n                      .\n                      <jats:italic>C. equi<\/jats:italic>\n                      and\n                      <jats:italic>C. hominis<\/jats:italic>\n                      were reported for the first time in Algerian horses and donkeys, respectively. Genotyping of\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      \u2010positive samples was unsuccessful due to sequencing failure, likely attributable to low parasite DNA concentrations.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study reports the first molecular prevalence data for\n                      <jats:italic>G. duodenalis<\/jats:italic>\n                      and the first detection of\n                      <jats:italic>C. equi<\/jats:italic>\n                      and\n                      <jats:italic>C. hominis<\/jats:italic>\n                      in Algerian equines. The zoonotic\n                      <jats:italic>C. parvum<\/jats:italic>\n                      subtype IIaA20G1R1, previously detected in Algerian HIV patients, highlights equines as a potential reservoir. Expanded One Health surveillance with longitudinal and seasonal sampling is recommended.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71083","type":"journal-article","created":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:17:27Z","timestamp":1784128647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence and Molecular Characterisation of\n                    <i>Cryptosporidium<\/i>\n                    spp. and\n                    <i>Giardia duodenalis<\/i>\n                    in Equines in Algeria"],"prefix":"10.1002","volume":"12","author":[{"given":"Nassiba","family":"Reghaissia","sequence":"first","affiliation":[{"name":"Laboratory of Sciences and Living Techniques Institute of Agronomic and Veterinary Sciences University of Souk Ahras  Souk Ahras Algeria"},{"name":"Laboratory of Exploration and Valorization of Steppic Ecosystems Faculty of Nature 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