{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:19:32Z","timestamp":1787919572784,"version":"build-2784847793"},"reference-count":37,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ageing is a progressive decline in organ function and tissue integrity caused by genetic, environmental and physiological factors. With the increasing lifespan of companion animals, effective anti\u2010ageing interventions for elderly felines and canines have attracted growing attention in veterinary medicine.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to investigate the anti\u2010ageing effects of green tea extract, keratin hydrolysate and vitamin C in feline and canine mammary gland cells and to explore the underlying molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Feline and canine mammary gland cells were treated with green tea extract, keratin hydrolysate and vitamin C in vitro. Cell proliferation, oxidative stress responses and ageing\u2010associated protein expression induced by actinomycin D were evaluated. The involvement of the Hippo signalling pathway was further analysed to determine the molecular mechanisms underlying the anti\u2010ageing effects.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Green tea extract, keratin hydrolysate and vitamin C significantly promoted the proliferation of feline and canine mammary gland cells. These treatments effectively restored the abnormal expression of ageing\u2010related proteins induced by actinomycin D and exhibited protective effects against oxidative damage. Mechanistically, the anti\u2010ageing effects were associated with regulation of the Hippo signalling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Green tea extract, keratin hydrolysate and vitamin C exhibit significant anti\u2010ageing effects in feline and canine mammary gland cells through modulation of the Hippo pathway. These findings suggest their potential as safe and effective anti\u2010ageing interventions for improving health and alleviating geriatric syndromes in ageing companion animals.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71088","type":"journal-article","created":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T07:33:25Z","timestamp":1784964805000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Green Tea Extract, Keratin Hydrolysate and Vitamin C Exert Anti\u2010Ageing Effects Through the Hippo Signalling Pathway in Feline and Canine Mammary Gland Cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Yi","family":"Gao","sequence":"first","affiliation":[{"name":"Nourse Centre for Pet Nutrition  Wuhu China"},{"name":"Shanghai Chowsing Pet Products Co., Ltd.  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Sun","sequence":"additional","affiliation":[{"name":"Nourse Centre for Pet Nutrition  Wuhu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Ma","sequence":"additional","affiliation":[{"name":"Shanghai Chowsing Pet Products Co., Ltd.  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxi","family":"Li","sequence":"additional","affiliation":[{"name":"Wuhu Weishi Biotechnology Co., Ltd.  Wuhu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunliang","family":"Li","sequence":"additional","affiliation":[{"name":"Nourse Centre for Pet Nutrition  Wuhu China"},{"name":"School of Food and Biological Engineering Jiangsu University  Zhenjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4820-3125","authenticated-orcid":false,"given":"Lei","family":"Lv","sequence":"additional","affiliation":[{"name":"Nourse Centre for Pet Nutrition  Wuhu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2023.115820"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.28410"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/nu9111211"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/23723556.2020.1791671"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mad.2020.111280"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/10826068.2013.763826"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2017.07.015"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcam.2020.100432"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(61)90192-6"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3233\/JAD-2012-120937"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2004.12.044"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yrtph.2018.03.019"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/D3RA03857B"},{"issue":"2","key":"e_1_2_11_15_1","first-page":"555","article-title":"Inhibitory Effect of Vitamin C on Intrinsic Aging in Human Dermal Fibroblasts and Hairless Mice","volume":"27","author":"Jeong J. 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