{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:19:17Z","timestamp":1787919557056,"version":"build-2784847793"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T00:00:00Z","timestamp":1784592000000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Anaplasmosis is a major tick\u2010borne infectious disease affecting small ruminants, leading to significant economic losses due to decreased productivity and, in some cases, mortality. This study aimed to determine the molecular prevalence of\n                    <jats:italic>Anaplasma ovis<\/jats:italic>\n                    and\n                    <jats:italic>Anaplasma phagocytophilum<\/jats:italic>\n                    infections in goats raised in Siirt province, T\u00fcrkiye, and to genetically characterize the detected isolates based on the major surface protein 4 (\n                    <jats:italic>MSP4<\/jats:italic>\n                    ) gene region. A total of 250 randomly selected apparently healthy goats were sampled between July and September 2025. The overall molecular prevalence of\n                    <jats:italic>A. ovis<\/jats:italic>\n                    was found to be 52% (130\/250), while\n                    <jats:italic>A. phagocytophilum<\/jats:italic>\n                    was not detected in any of the screened animals. Significant associations were observed between\n                    <jats:italic>A. ovis<\/jats:italic>\n                    positivity and age, sex, breed, and sampling districts within Siirt province (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.05), with the highest infection rates observed in goats older than 1 year (63.6%), female goats (58.2%), Damascus goats (66.7%), and goats sampled from the Kurtalan district (87.1%). Sequencing analysis of selected samples yielded high\u2010quality\n                    <jats:italic>MSP4<\/jats:italic>\n                    gene sequences showing 100% nucleotide identity with reference\n                    <jats:italic>A. ovis<\/jats:italic>\n                    isolates from Iran, Italy, Pakistan, Sudan, Tunisia, and T\u00fcrkiye. Phylogenetic analysis supported species\u2010level identification by grouping the study isolates with reference\n                    <jats:italic>A. ovis<\/jats:italic>\n                    sequences; however, broader evolutionary inferences should be interpreted cautiously due to the short fragment length and limited number of sequenced isolates. The findings of this study provide updated molecular epidemiological data on caprine anaplasmosis in southeastern T\u00fcrkiye and highlight the utility of the\n                    <jats:italic>MSP4<\/jats:italic>\n                    gene as a reliable molecular marker for species identification and phylogenetic analysis. These results contribute to a better understanding of the epidemiology of\n                    <jats:italic>A. ovis<\/jats:italic>\n                    and may support the development of effective control strategies in small ruminant production systems.\n                  <\/jats:p>","DOI":"10.1002\/vms3.71093","type":"journal-article","created":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T17:54:53Z","timestamp":1784656493000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Characterization and Prevalence of\n                    <i>Anaplasma ovis<\/i>\n                    and\n                    <i>Anaplasma phagocytophilum<\/i>\n                    in Goat Population in Siirt Province From T\u00fcrkiye"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1769-4558","authenticated-orcid":false,"given":"Muhammed Ahmed","family":"Selcuk","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Parasitology Siirt University  Siirt T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamer","family":"Karaku\u015f","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Parasitology Siirt University  Siirt T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rustu Erdem","family":"Ozen","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Parasitology Siirt University  Siirt T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8072-2983","authenticated-orcid":false,"given":"Ceylan","family":"Ceylan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Parasitology Siirt University  Siirt T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3514-5221","authenticated-orcid":false,"given":"Onur","family":"Ceylan","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Parasitology Selcuk University  Konya T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,21]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"367","article-title":"Molecular Identification of Anaplasmosis in Goats Using a New PCR\u2010RFLP Method","volume":"10","author":"Ahmadi\u2010Hamedani M.","year":"2009","journal-title":"Iranian Journal of Veterinary 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