{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:54:36Z","timestamp":1791204876047,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T00:00:00Z","timestamp":1786492800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Rapid and reliable diagnostic methods suitable for field use are needed for the detection of\n                      <jats:italic>Erysipelothrix rhusiopathiae<\/jats:italic>\n                      in poultry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      This study aims to develop and evaluate a colourimetric loop\u2010mediated isothermal amplification (LAMP) assay for the rapid detection of\n                      <jats:italic>E. rhusiopathiae<\/jats:italic>\n                      in conjunction with a rapid DNA extraction procedure suitable for field conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Specific primers targeting a conserved region of a putative polypeptide gene involved in the synthesis of capsular polysaccharides were used for LAMP and PCR. The specificity of the developed assays was evaluated using 13 unrelated bacterial strains. The efficacy of the assays was assessed using positive controls and 52 clinical samples collected from poultry farms experiencing erysipelas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both LAMP and PCR showed high sensitivity and specificity when compared with bacterial culture. LAMP and PCR achieved 100% sensitivity and specificity when combined with the rapid DNA extraction method. Using a commercial DNA extraction kit, LAMP maintained 100% sensitivity and specificity, whereas PCR showed 100% sensitivity and 97.8% specificity. The high correlation observed between bacterial culture and the LAMP assay demonstrated the effectiveness and reliability of the rapid DNA extraction method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The developed colourimetric LAMP assay provides a rapid, sensitive, and practical diagnostic tool for the detection of\n                      <jats:italic>E. rhusiopathiae<\/jats:italic>\n                      in poultry. The assay requires minimal instrumentation, enables straightforward visual interpretation of results, and is suitable for point\u2010of\u2010care testing and resource\u2010limited settings.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71100","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T17:47:03Z","timestamp":1786556823000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Development and Evaluation of a Colourimetric LAMP Assay for Field Detection of\n                    <i>Erysipelothrix rhusiopathiae<\/i>\n                    in Poultry Using a Rapid DNA Extraction Method"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9820-5308","authenticated-orcid":false,"given":"Seyed Ali","family":"Ghorashi","sequence":"first","affiliation":[{"name":"School of Agricultural Environmental and Veterinary Sciences, Charles Sturt University  Wagga Wagga Australia"},{"name":"Gulbali Institute Charles Sturt University  Wagga Wagga Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiongrui","family":"Huang","sequence":"additional","affiliation":[{"name":"Scolexia Pty Ltd.  Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Scott","sequence":"additional","affiliation":[{"name":"Scolexia Pty Ltd.  Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameer D.","family":"Pant","sequence":"additional","affiliation":[{"name":"School of Agricultural Environmental and Veterinary Sciences, Charles Sturt University  Wagga Wagga Australia"},{"name":"Gulbali Institute Charles Sturt University  Wagga Wagga Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0185340"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinmicnews.2015.09.001"},{"key":"e_1_2_11_4_1","first-page":"1010","volume-title":"Diseases of Poultry","author":"Eriksson H.","year":"2019"},{"key":"e_1_2_11_5_1","article-title":"Comparison of PCR\u2010HRM, Colorimetric LAMP and Culture Based Diagnostic Assays in the Detection of Endometritis Caused by Streptococcus Equi subsp. zooepidemicus in Mares","author":"Garner C.","year":"2022","journal-title":"Veterinary Research Communications"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001572"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_2_11_8_1","unstructured":"Papadakis G. A. K.Pantazis N.Fikas et\u00a0al.2021. \u201cPortable Real\u2010Time Colorimetric LAMP\u2010Device for Rapid Quantitative Detection Of Nucleic Acids in Crude Samples.\u201d bioRxiv.https:\/\/www.biorxiv.org\/content\/biorxiv\/early\/2021\/05\/27\/2020.07.22.215251.full.pdf."},{"key":"e_1_2_11_9_1","article-title":"Comparative Evaluation of PCR and Loop\u2010Mediated Isothermal Amplification (LAMP) Assays for Detecting Pasteurella multocida in Poultry","author":"Poussard M.","year":"2024","journal-title":"New Zealand Veterinary Journal"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.71.471"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.2144\/00291bm09"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.609821"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/lam.12198"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2144\/btn-2020-0078"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2023.1183360"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.71100","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.71100","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.71100","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:26:44Z","timestamp":1791203204000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.71100"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,12]]},"references-count":14,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,9]]}},"alternative-id":["10.1002\/vms3.71100"],"URL":"https:\/\/doi.org\/10.1002\/vms3.71100","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.71100\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.71100\/v1\/decision1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,12]]},"assertion":[{"value":"2025-07-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-04","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71100"}}