{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:54:10Z","timestamp":1791204850952,"version":"4.1.0"},"reference-count":45,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Neutrophil extracellular traps (NETs) have gained attention in oncology due to their role in inflammation\u2010driven tumour progression. While their involvement has been documented in various human malignancies, including select sarcoma subtypes, data on soft tissue sarcomas (STS) remain sparse, particularly in veterinary species.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to investigate the presence and potential significance of NET formation in spontaneously occurring canine STS, with an additional focus on possible translational relevance to human oncology.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>This study examined 30 canine soft tissue tumours, including fibroma, leiomyoma, fibromyxosarcoma, fibrosarcoma, hemangiosarcoma, liposarcoma, undifferentiated pleomorphic sarcoma, myxosarcoma, perivascular wall tumours, peripheral nerve sheath tumours and undifferentiated sarcoma. NET formations (including both NET\u2010primed neutrophils and NETs) were detected through a dual\u2010staining technique using citrullinated histone\u20103 (citH3) and myeloperoxidase antibodies on the same tissue sections, employing a fading and restaining method. NET formation was quantified based on citH3\u2010positive areas and analysed against tumour grade and other histopathological markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Pearson correlation analysis found no significant association between NET scores and tumour grade, mitotic activity, necrosis or differentiation. However, NET formation was significantly elevated in malignant sarcomas compared to benign tumours. Within sarcomas, grade III tumours exhibited slightly higher NET formation, though the difference was not statistically significant relative to grades I and II.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings parallel emerging evidence in human STS, where NETs have been implicated in tumour progression, though their precise role remains underexplored. By utilizing spontaneous canine sarcomas as a translational model, this study contributes to a deeper understanding of NET\u2010driven tumour biology and may help potential diagnostic or therapeutic strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71123","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T14:43:18Z","timestamp":1785508998000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Exploring Neutrophil Extracellular Traps in Canine Soft Tissue Sarcomas: A Preliminary Study With Translational Insights"],"prefix":"10.1002","volume":"12","author":[{"given":"Burak","family":"Yildirim","sequence":"first","affiliation":[{"name":"Health Sciences Institute Burdur Mehmet Akif Ersoy University  Burdur T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5874-7797","authenticated-orcid":false,"given":"Volkan","family":"Ipek","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Pathology Burdur Mehmet Akif Ersoy University  Burdur T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1354\/vp.44\u20105\u2010607"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKY1049"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00048"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a037028"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13112832"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0011768"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300985810388820"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010018\u201020479\u2010x"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.5326\/0410241"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968\u20104328(99)00064\u20105"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2009.953612"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470752487"},{"key":"e_1_2_12_14_1","first-page":"29","volume-title":"World Health Organization Histological Classification of Tumors of Domestic Animals","author":"Hendrick M. 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