{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:54:27Z","timestamp":1791204867439,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cryopreservation induces oxidative stress, leading to reduced semen quality in canine semen. Clinoptilolite (CLP), a natural zeolite, has potential antioxidant properties that may improve post\u2010thaw sperm parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to evaluate the dose\u2010dependent effects of CLP supplementation in semen extenders on sperm quality and oxidative stress in dogs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Semen was collected from four adult dogs by manual manipulation, divided into five equal aliquots, and diluted at a ratio of 1:3 (v\/v) with a Tris\u2013citrate extender containing 0% (control), 1%, 2%, 3%, or 4% CLP. Samples were cooled to 5\u00b0C within 45 min, equilibrated for 2 h in 0.25\u00a0mL straws, frozen in liquid nitrogen vapour, and stored in liquid nitrogen. Samples were thawed at 37\u00b0C for 30 s. Post\u2010thaw sperm quality was evaluated together with oxidative stress markers, including total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The 2% CLP group showed the highest post\u2010thaw sperm quality, whereas the lowest performance was observed in the 4% CLP group. Additionally, the 2% CLP group exhibited a more favourable oxidative profile, with higher TAS and lower TOS and OSI values than all other groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CLP supplementation exerted dose\u2010dependent effects on sperm quality and oxidative balance in canine semen cryopreservation. A 2% CLP concentration appears to be the most effective for improving post\u2010thaw sperm quality.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71128","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T08:52:04Z","timestamp":1785919924000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dose\u2010Dependent Effects of Clinoptilolite Supplementation on Sperm Quality and Oxidative Stress During Canine Semen Cryopreservation"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7120-8146","authenticated-orcid":false,"given":"Nurdan","family":"Co\u015fkun","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination Hatay Mustafa Kemal University  Hatay Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1765-4655","authenticated-orcid":false,"given":"Fikret","family":"Karaca","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Reproduction and Artificial Insemination Hatay Mustafa Kemal University  Hatay Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1071\/RD03089"},{"key":"e_1_2_11_3_1","volume-title":"Solid\u2010State Supramolecular Chemistry: Two\u2010 and Three\u2010Dimensional Inorganic Networks","author":"Alberti G.","year":"1996"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules22071107"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0093-691X(01)00590-8"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smallrumres.2012.02.002"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2003.11.015"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2005.08.008"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2019.10.010"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119265306"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2004.11.022"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02850340"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijvsm.2017.11.001"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.01350"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-7500-8_14"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24081517"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.21608\/jappmu.2019.63457"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cryobiol.2021.08.005"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0531.2009.01434.x"},{"issue":"2","key":"e_1_2_11_20_1","first-page":"9","article-title":"Effect of Zeolite (Clinoptilolite) as a Salinity Stress Alleviator on Semen Quality and Hemato\u2010Biochemical Parameters of Montazah Cocks Under South Sinai Conditions","volume":"10","author":"Morsy A. 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