{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:54:24Z","timestamp":1791204864074,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004951","name":"Atat\u00fcrk \u00dcniversitesi","doi-asserted-by":"publisher","award":["TDK\u20102023\u201013014"],"award-info":[{"award-number":["TDK\u20102023\u201013014"]}],"id":[{"id":"10.13039\/501100004951","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Laparoscopic ovariectomy (LOE) is a common procedure for elective sterilization in dogs and is associated with less tissue trauma than open techniques. However, manipulation of the ovarian pedicle may still induce nociceptive stimulation and contribute to perioperative inflammatory responses and changes in antioxidant enzyme activity. Topical administration of local anaesthetic to the ovarian pedicle may improve perioperative analgesia in minimally invasive surgery.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To evaluate the effects of topical administration of bupivacaine HCl to the ovarian pedicle on postoperative pain, serum antioxidant enzyme activity and systemic pro\u2010inflammatory cytokines in dogs undergoing LOE.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010four clinically healthy, American Society of Anaesthesiologists (ASA) Class I female dogs were enrolled in a prospective, randomized, controlled trial. The dogs were allocated to receive either a total dose of bupivacaine HCl at 2.2\u00a0mg\/kg (BPLOVE,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a012), divided between the two ovarian pedicles, or an equivalent volume of isotonic saline (LOVE,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a012), applied using the same topical technique. Standardized anaesthesia and surgical protocols were implemented. Postoperative pain was assessed using the University of Melbourne Pain Scale (UMPS) at 0, 2 and 4\u00a0h after surgery. Serum samples collected preoperatively, intraoperatively, and at 0, 2 and 4\u00a0h postoperatively were analysed for superoxide dismutase (SOD), catalase (CAT), interleukin\u20101\u03b2 (IL\u20101\u03b2), interleukin\u20106 (IL\u20106) and tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1). Data were analysed using repeated\u2010measures ANOVA and non\u2010parametric tests (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All dogs completed the study without experiencing any major complications or requiring intraoperative rescue analgesia. UMPS scores were significantly lower in the BPLOVE group at all postoperative time points (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Significant group and time effects were observed in SOD and CAT activities, with higher antioxidant enzyme activity detected in bupivacaine\u2010treated dogs (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). Cytokine outcomes showed no consistent between\u2010group differences; IL\u20106 was numerically lower in the BPLOVE group across time points, with a significant difference detected only at 4\u00a0h postoperatively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Topical administration of bupivacaine HCl to the ovarian pedicle during LOE was associated with improved early postoperative analgesia and higher serum SOD and CAT activities, without consistent short\u2010term changes in systemic pro\u2010inflammatory cytokine concentrations. These findings support the potential value of ovarian pedicle bupivacaine administration as part of multimodal analgesia in canine laparoscopic surgery and indicate changes in antioxidant enzyme activity during the early perioperative period.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71144","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T09:32:08Z","timestamp":1785922328000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of Topical Administration of Bupivacaine HCl to the Ovarian Pedicle on Postoperative Pain, Antioxidant Enzyme Activity and Inflammatory Cytokines in Dogs Undergoing Laparoscopic Ovariectomy"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7912-7703","authenticated-orcid":false,"given":"Alper Yasin","family":"\u00c7iplak","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine Atat\u00fcrk University  Erzurum Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1790-6808","authenticated-orcid":false,"given":"B\u00fclent","family":"Polat","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Faculty of Veterinary Medicine Atat\u00fcrk University  Erzurum Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010025\u201004671\u20106"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2024.2379319"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467\u20102995.2008.00421.x"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/vsu.70070"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.1031345"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.637"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaa.2016.12.059"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani15162336"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14111349"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani13233604"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11259\u2010025\u201010969\u20105"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.7759\/cureus.94719"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ani12243556"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2024.106156"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0009\u20108981(91)90067\u2010M"},{"key":"e_1_2_13_17_1","first-page":"1","article-title":"Optimal Multimodal Analgesia Combinations to Reduce Pain and Opioid Use Following Non\u2010Cardiac Surgery: An Instrumental Variable Analysis","author":"Graham L. 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