{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T18:39:44Z","timestamp":1792348784609,"version":"4.4.0"},"reference-count":75,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004240","name":"Akademie V\u011bd \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["PPLZ L200451951"],"award-info":[{"award-number":["PPLZ L200451951"]}],"id":[{"id":"10.13039\/501100004240","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Ex situ conservation of threatened felid species requires a thorough understanding of reproductive biology and realistic evaluation of assisted reproductive techniques (ART). In jaguars (\n                      <jats:italic>Panthera onca<\/jats:italic>\n                      ), however, knowledge of reproductive limitations in captivity remains scarce.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>On the basis of a comprehensive single\u2010timepoint assessment, this study applied an integrated clinical, endocrinological, semen analysis and cytogenetic approach to investigate persistent infertility in a captive jaguar breeding pair recommended for reproduction within the European Association of Zoos and Aquaria (EAZA) programme.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Both animals underwent comprehensive reproductive and health assessments, including genital examination, ultrasonography, haematological and hormonal profiling. Semen quality was evaluated following electroejaculation, and cytogenetic screening was performed to detect potential chromosomal abnormalities using conventional staining, banding techniques and fluorescence in situ hybridization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Cytogenetic analyses revealed a standard felid karyotype (2\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a038) in both individuals, with no evidence of aneuploidy or structural chromosomal rearrangements. Clinical and endocrinological assessments of the female did not identify abnormalities likely to impair fertility. In contrast, semen evaluation of the male revealed markedly reduced ejaculate volume, low sperm concentration, low sperm motility and a high proportion of morphologically abnormal spermatozoa, consistent with severe teratozoospermia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Taken together, these findings indicate that although genetic and general clinical contraindications for reproduction were absent, semen quality represents a critical limiting factor for successful natural breeding. Under the observed reproductive profile, ART feasibility requires functional validation through sperm selection and competence assays. This study highlights the value of combining cytogenetic and reproductive assessments to support evidence\u2010based decision\u2010making in captive breeding programmes and provides novel cytogenetic data for the jaguar.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71192","type":"journal-article","created":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T17:46:26Z","timestamp":1792345586000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated Reproductive and Cytogenetic Assessment of Infertility in a Captive Jaguar (\n                    <i>Panthera onca<\/i>\n                    )"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7193-8918","authenticated-orcid":false,"given":"Marie","family":"Altmanov\u00e1","sequence":"first","affiliation":[{"name":"Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics Czech Academy of Sciences  Lib\u011bchov Czech Republic"},{"name":"Department of Ecology Faculty of Science Charles University  Prague Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Strand","sequence":"additional","affiliation":[{"name":"Department of Animal and Veterinary Sciences \u2010 ANIVET Gut and host health Aarhus University Aarhus Denmark"},{"name":"Randers Regnskov  Randers Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathrine Thejll","family":"Kirchhoff","sequence":"additional","affiliation":[{"name":"Evidensia Dania Hinnerup Dyrehospital Hinnerup Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7100-2922","authenticated-orcid":false,"given":"Zuzana","family":"Majt\u00e1nov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics Czech Academy of Sciences  Lib\u011bchov Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,18]]},"reference":[{"key":"e_1_2_12_2_1","volume-title":"EAZA Conservation Programmes","author":"European Association of Zoos and Aquaria","year":"2019"},{"key":"e_1_2_12_3_1","volume-title":"Jaguar (Panthera onca) Care Manual","author":"AZA Jaguar Species Survival Plan","year":"2026"},{"key":"e_1_2_12_4_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ygcen.2015.09.012","article-title":"Characterization and Manipulation of Reproductive Cycles in the Jaguar (Panthera onca)","volume":"225","author":"Barnes S. 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