{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:54:48Z","timestamp":1791204888390,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T00:00:00Z","timestamp":1787961600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Mastitis in dairy animals is a major economic and public health concern in Nepal, exacerbated by the emergence of antimicrobial\u2010resistant pathogens. This study aimed to determine the prevalence and antibiotic resistance patterns of\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      isolated from bovine milk in Bardaghat Municipality, Nepal.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional survey screened 196 milk samples from lactating cows and buffaloes using the California Mastitis Test (CMT). The overall prevalence of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      was 39.79%. Strikingly, 100.00% of isolates exhibited resistance to oxacillin, indicating the widespread presence of methicillin\u2010resistant\n                      <jats:italic>Staphylococcus aureus<\/jats:italic>\n                      (MRSA). Moreover, 25.64% of isolates were multidrug\u2010resistant (resistant to n\u00a03 antibiotic classes), and 18% had a multiple antibiotic resistance (MAR) index &gt;\u00a00.2. No statistically significant associations were found between the prevalence of\n                      <jats:italic>S. aureus<\/jats:italic>\n                      and evaluated risk factors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings underscore an urgent need for improved mastitis management, including routine bacterial screening, judicious antibiotic use and public health measures to mitigate transmission of resistant\n                      <jats:italic>S. aureus<\/jats:italic>\n                      to humans.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71204","type":"journal-article","created":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T17:36:14Z","timestamp":1788024974000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Antibiogram of\n                    <i>Staphylococcus aureus<\/i>\n                    Isolated From Bovine Mastitis in Bardaghat Municipality, Nepal"],"prefix":"10.1002","volume":"12","author":[{"given":"Biswash","family":"Ghimire","sequence":"first","affiliation":[{"name":"Paklihawa Campus Institute of Agriculture and Animal Science Tribhuvan University  Rupandehi Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7675-8657","authenticated-orcid":false,"given":"Shambhu","family":"Shah","sequence":"additional","affiliation":[{"name":"Paklihawa Campus Institute of Agriculture and Animal Science Tribhuvan University  Rupandehi Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7838-8442","authenticated-orcid":false,"given":"Akash","family":"Adhikari","sequence":"additional","affiliation":[{"name":"Paklihawa Campus Institute of Agriculture and Animal Science Tribhuvan University  Rupandehi Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-6097-9486","authenticated-orcid":false,"given":"Sujan","family":"Adhikari","sequence":"additional","affiliation":[{"name":"Faculty of Animal Science Veterinary Science and Fisheries Agriculture and Forestry University  Rampur Chitwan Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmita","family":"Regmi","sequence":"additional","affiliation":[{"name":"Paklihawa Campus Institute of Agriculture and Animal Science Tribhuvan University  Rupandehi Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3064-3173","authenticated-orcid":false,"given":"Sirjan","family":"Bastola","sequence":"additional","affiliation":[{"name":"Paklihawa Campus Institute of Agriculture and Animal Science Tribhuvan University  Rupandehi Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,29]]},"reference":[{"key":"e_1_2_14_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiaa031"},{"key":"e_1_2_14_3_1","doi-asserted-by":"publisher","DOI":"10.22099\/IJVR.2023.43941.6445"},{"key":"e_1_2_14_4_1","first-page":"160","article-title":"Identification of Coagulase Gene in Staphylococcus aureus Isolates Recovered From Subclinical Mastitis in Camels","volume":"37","author":"Aqib A. 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