{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:07:12Z","timestamp":1790690832390,"version":"4.1.0"},"reference-count":77,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The extensive use of pesticides in agriculture has increased their runoff into aquatic ecosystems, posing serious risks to non\u2010target organisms. Fish are recognized as sensitive bioindicators and are widely used to evaluate the ecological impacts of pesticide contamination.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      This study investigated the acute toxic effects of bifenthrin + chlorpyrifos (B + C) and bifenthrin + endosulfan (B + E) mixtures on antioxidant enzyme activities, DNA damage and nuclear abnormalities (NAs) in\n                      <jats:italic>Catla catla<\/jats:italic>\n                      and\n                      <jats:italic>Labeo rohita<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Acute toxicity tests were conducted to determine the 96\u2010h LC\n                      <jats:sub>50<\/jats:sub>\n                      values of both pesticide mixtures. Fish were subsequently exposed to the respective LC\n                      <jats:sub>50<\/jats:sub>\n                      concentrations for 4 days, with sampling at 24\u2010h intervals. Antioxidant enzymes, including superoxide dismutase (SOD), glutathione S\u2010transferase (GST), peroxidase (POx) and catalase (CAT), were evaluated in different organs. Genotoxic effects were assessed through micronucleus (MN), NAs, damaged nuclei and genomic damage index (GDI) analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The 96\u2010h LC\n                      <jats:sub>50<\/jats:sub>\n                      values of the B + E mixture were 1.86 \u00b1 0.01 and 2.84 \u00b1 0.02 \u00b5g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      , whereas those of the B + C mixture were 2.09 \u00b1 0.01 and 3.41 \u00b1 0.07 \u00b5g L\n                      <jats:sup>\u22121<\/jats:sup>\n                      for\n                      <jats:italic>C. catla<\/jats:italic>\n                      and\n                      <jats:italic>L. rohita<\/jats:italic>\n                      , respectively.\n                      <jats:italic>C. catla<\/jats:italic>\n                      exhibited greater sensitivity to both mixtures. SOD and GST activities increased significantly (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05) in all organs with increasing exposure duration. POx activity generally increased with exposure time, while CAT activity increased in gills, liver and kidney but declined in brain, heart and muscle tissues. Micronuclei and other NAs increased significantly with exposure duration in both species. Genomic damage indices showed significant positive dependence on exposure duration in\n                      <jats:italic>L. rohita<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Combined exposure to bifenthrin\u2013chlorpyrifos and bifenthrin\u2013endosulfan mixtures induces pronounced oxidative stress and genotoxic damage in\n                      <jats:italic>C. catla<\/jats:italic>\n                      and\n                      <jats:italic>L. rohita<\/jats:italic>\n                      , highlighting the usefulness of antioxidant and DNA damage biomarkers for monitoring pesticide contamination in aquatic environments.\n                    <\/jats:p>\n                  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