{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:53:28Z","timestamp":1791986008162,"version":"4.4.0"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Double\u2010antibody sandwich enzyme\u2010linked immunosorbent assay (DAS\u2010ELISA) is a simple and accurate method for detecting viral pathogens. This study established a DAS\u2010ELISA for the diagnosis of feline coronavirus (FCoV).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, DAS\u2010ELISA was performed using FCoV S\u2010protein rabbit polyclonal and mouse monoclonal antibodies. The optimal reaction conditions were determined using the chessboard method. The specificity, repeatability and receiver operating characteristic (ROC) curve were used to verify the clinical application potential of the DAS\u2010ELISA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The intra\u2010 and inter\u2010assay %CVs were 3.90\u201314.10 and 2.55\u201311.02, respectively. In a preliminary specificity evaluation using 12 faecal samples each from cats infected with feline panleukopenia virus, feline calicivirus or feline herpesvirus, the DAS\u2010ELISA showed no detectable cross\u2010reactivity with any of these pathogens. The positive coincidence rate of DAS\u2010ELISA and RT\u2010PCR was 81.3%. The ROC curve showed that the sensitivity, specificity and ROC AUC were 81.3%, 80.0% and 0.861, respectively.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Collectively, these results suggest that the developed DAS\u2010ELISA can be used to detect FCoV pathogens in clinical samples. This study provides a selection method for the diagnosis of FCoV and a data supplement for epidemiological studies of FCoV.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.71289","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T13:37:09Z","timestamp":1791985029000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Double\u2010Antibody Sandwich Enzyme\u2010Linked Immunosorbent Assay for Detecting Feline Coronavirus Infection"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1785-7559","authenticated-orcid":false,"given":"Bo","family":"Dong","sequence":"first","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"},{"name":"Engineering Research Center for the Prevention and Control of Animal Original Zoonosis College of Life Sciences Fujian Province University Longyan University  Longyan China"},{"name":"Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijie","family":"Zou","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqian","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxiao","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqing","family":"Jing","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuquan","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7780-526X","authenticated-orcid":false,"given":"Weiming","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Life Sciences of Longyan University  Longyan China"},{"name":"Engineering Research Center for the Prevention and Control of Animal Original Zoonosis College of Life Sciences Fujian Province University Longyan University  Longyan China"},{"name":"Fujian Provincial Key Laboratory for the Prevention and Control of Animal Infectious Diseases and Biotechnology  Longyan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"issue":"1","key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1099\/0022-1317-59-1-23","article-title":"The Ultrastructure of Feline Infectious Peritonitis Virus in Feline Embryonic Lung Cells","volume":"59","author":"Beesley J. 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