{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:42:33Z","timestamp":1790520153183,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T00:00:00Z","timestamp":1644019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Brucellosis, as a zoonotic disease, mainly occurs in horses by\n                      <jats:italic>Brucella abortus, Brucella canis<\/jats:italic>\n                      and\n                      <jats:italic>Brucella suis<\/jats:italic>\n                      . The disease in equines is often asymptomatic, but the clinical signs in horses are mostly characterized by bursitis, arthritis and tenosynovitis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study, thus, aimed to determine the seroprevalence of brucellosis and its associated risk factors in the Arabian horses of Khuzestan province, South\u2010west Iran.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To that end, the blood samples randomly collected from 180 Arabian horses were analyzed for the presence of anti\u2010\n                      <jats:italic>Brucella<\/jats:italic>\n                      antibodies by Rose Bengal plate test (RBPT), serum agglutination test (SAT), 2\u2010mercaptoethanol test (2\u2010ME) and a commercial i\u2010ELISA kit.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The ROC curve analysis showed that the best cut\u2010off point for\n                      <jats:italic>S\/P<\/jats:italic>\n                      values in i\u2010ELISA turned out to be 26.25%. The results showed that the overall seroprevalence of brucellosis based on parallel interpretation of the test results was 12.22% (Positive\/Tested\u00a0=\u00a022\/180). The prevalence of acute and chronic brucellosis was 8.3 and 3.9%, respectively. The seroprevalence of brucellosis with RBPT and i\u2010ELISA methods was 1.11% (2\/180) and 7.22% (13\/180), respectively. According to what SAT revealed, 9.44% (17\/180) of sera had a titer of 40 or greater, and at 2\u2010ME, 7.22% of samples (13 out of 180 samples) depicted a titer of 40. The results of i\u2010ELISA, SAT and 2\u2010ME were significantly different from those of RBPT (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &lt;\u00a00.01); however, there was no significant difference between i\u2010ELISA, SAT and 2\u2010ME in findings (\n                      <jats:italic>p\u00a0<\/jats:italic>\n                      &gt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results of this study recommend that i\u2010ELISA be used for screening purposes of brucellosis in horses. The findings confirmed that Arabian horses are natural hosts for the Brucellae. It is, thus, necessary to adopt appropriate prevention and control programs by health authorities and horse owners so as to reduce the distribution and transmission of the infection in the regions where brucellosis is prevalent.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.759","type":"journal-article","created":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T10:39:40Z","timestamp":1644057580000},"page":"1056-1064","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Seroprevalence and risk factors of brucellosis in Arabian horses"],"prefix":"10.1002","volume":"8","author":[{"given":"Zahra","family":"Lotfi","sequence":"first","affiliation":[{"name":"Department of Food Hygiene Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz Ahvaz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9478-260X","authenticated-orcid":false,"given":"Mahdi","family":"Pourmahdi Borujeni","sequence":"additional","affiliation":[{"name":"Department of Food Hygiene Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz Ahvaz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"Ghorbanpoor","sequence":"additional","affiliation":[{"name":"Department of Pathobiology Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz Ahvaz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Reza","family":"Ghadrdan Mashhadi","sequence":"additional","affiliation":[{"name":"Department of Clinical Sciences Faculty of Veterinary Medicine Shahid Chamran University of Ahvaz Ahvaz Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,5]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/vr.165.9.267"},{"key":"e_1_2_11_3_1","first-page":"302","article-title":"Prevalence of Brucella abortus antibodies in equines of a tropical region of Mexico","volume":"70","author":"Acosta\u2010Gonzalez R. 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