{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:59Z","timestamp":1772118959902,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T00:00:00Z","timestamp":1652140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aimed to describe the technique and clinical outcomes in dogs undergoing Laparoscopic Adipose\u2010Derived Stem Cell Harvest via bipolar sealing device (LADSCHB) for degenerative orthopaedic and neurologic disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design<\/jats:title>\n                    <jats:p>Descriptive retrospective case series.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Animals<\/jats:title>\n                    <jats:p>Eleven dogs with orthopaedic disease and one dog with degenerative spinal disease were enrolled in the study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Medical records of dogs undergoing LADSCHB were reviewed for signalment, weight, reason for the procedure, anaesthesia time, surgery time, other procedures performed, post\u2010operative pain protocols, incision size, amount of adipose tissue collected, number of viable cells collected, days to discharge, short\u2010term complications, and owner satisfaction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The median weight of the population was 34.2\u00a0kg (range 9.2\u201362\u00a0kg), the median surgery time was 39 min (range 15\u201345 min), mean incision length was 2.5\u00a0cm, the median amount of adipose collected was 60\u00a0g, and the median number of viable stem cells was 21 million cells. Conversion to open laparotomy was not needed. The most common reason for the harvest was osteoarthritis of the elbow (8\/12 cases). Nine cases had other procedures performed at the same time as the harvest. No complications were noted during the procedure or within the post\u2010operative period. All owners surveyed were satisfied with the laparoscopic harvest procedure.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>LADSCHB was technically feasible, productive, and not associated with any complications. This procedure was performed rapidly and was paired with other surgical procedures.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Clinical Significance<\/jats:title>\n                    <jats:p>LADSCHB allows for stem cell harvest with commonly utilized laparoscopic equipment. This surgical technique could lead to the increased ability to treat patients with diseases that benefit from stem cell therapy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.816","type":"journal-article","created":{"date-parts":[[2022,5,10]],"date-time":"2022-05-10T15:52:33Z","timestamp":1652197953000},"page":"1421-1428","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Laparoscopic adipose\u2010derived stem cell harvest technique with bipolar sealing device: Outcome in 12 dogs"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4623-3582","authenticated-orcid":false,"given":"Nicole J.","family":"Buote","sequence":"first","affiliation":[{"name":"VCA West Los Angeles Animal Hospital  Los Angeles California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,10]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"177","article-title":"Patient factors influencing the concentration of stromal vascular fraction (SVF) for adipose\u2010derived stromal cell (ASC) therapy in dogs","volume":"77","author":"Astor D. 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