{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:26Z","timestamp":1772118926002,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Zoonotic visceral leishmaniasis (VL) is caused by\n                      <jats:italic>Leishmania infantum<\/jats:italic>\n                      , of which dogs are the main reservoir. VL is endemic in the Middle East, also in some parts of Iran. Following reports of new cases of VL in children in Maragheh County, the non\u2010endemic area of the disease, we encouraged to conduct a preliminary study on domestic dogs and cats to identify their potential role as reservoirs for the disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      This study was conducted during a period of 3 years from 2018 to 2021. Two hundred ownership dogs and 25 cats from Maragheh County, north\u2010west of Iran, were randomly screened. Blood samples were collected. A direct agglutination test (DAT) was used for the detection of anti\u2010\n                      <jats:italic>L. infantum<\/jats:italic>\n                      antibodies. Furthermore, buffy coat samples from the\n                      <jats:italic>L. infantum<\/jats:italic>\n                      seropositive animals were examined to detect parasite presence using polymerase chain reaction.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Out of the total of 200 ownership dogs evaluated, 170 (85%) were male and 30 (15%) were female with a mean age of 4.3\u00a0years. Anti\u2010\n                      <jats:italic>L. infantum<\/jats:italic>\n                      antibodies (IgG cut\u2010off\u00a0\u2265\u00a01:320) were observed in 3.5% of dogs (7\/200) by the DAT test. All seropositive dogs were identified in the first year of examination. Regarding molecular approaches in seropositive dogs, two samples were positive for a 565 bp kDNA minicircle gene specific for\n                      <jats:italic>L. infantum<\/jats:italic>\n                      . During the study period, no seropositive case was detected in the cats examined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study shows that the domestic cycle of\n                      <jats:italic>L. infantum<\/jats:italic>\n                      has been established in the studied region. It is necessary to increase the awareness and monitoring of the disease with the study of wild reservoirs periodically.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.846","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T13:28:47Z","timestamp":1653658127000},"page":"1898-1903","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular and serological evaluation of visceral leishmaniasis in domestic dogs and cats in Maragheh County, north\u2010west of Iran, 2018\u20132021"],"prefix":"10.1002","volume":"8","author":[{"given":"Ali","family":"Soleimani","sequence":"first","affiliation":[{"name":"Department of public health Maragheh University of Medical Sciences  Maragheh Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[{"name":"Deptaetment of Medical Parasitology and Mycology School of Public Health Tehran University of Medical Sciences  Tehran Iran"},{"name":"Center for Research of Endemic Parasites of Iran (CREPI) Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saber","family":"Gholizadeh","sequence":"additional","affiliation":[{"name":"Department of Medical Entomology School of Public Health Urmia University of Medical Sciences  Urmia Iran"},{"name":"Social Determinants of Health Research Center Clinical Research Institute Urmia University of Medical Sciences  Urmia Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arezoo","family":"Bozorgomid","sequence":"additional","affiliation":[{"name":"Infectious Diseases Research Center Kermanshah University of Medical Sciences  Kermanshah Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Shafiei","sequence":"additional","affiliation":[{"name":"Vector\u2010borne Diseases Research Center North Khorasan University of Medical Sciences  Bojnurd Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8283-6447","authenticated-orcid":false,"given":"Saber","family":"Raeghi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Sciences Maragheh University of Medical Sciences  Maragheh Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,5,27]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-9199-jvatitd-2019-0012"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-013-0325-2"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.4103\/1995-7645.262074"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004987"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2021.105638"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.10.2252-2257.1989"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-014-0559-7"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12639-014-0615-3"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2009.0023"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/0036554021000026930"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2012.10.013"},{"key":"e_1_2_11_13_1","unstructured":"Mohebali M. Edrisian G. H. Nadim A. Hajaran H. Akhoundi B. Houshmand B. Zareei Z. Arshi S. Mirsamadi N. &Manouchehri N. K.(2006).Application of direct agglutination test (DAT) for the diagnosis and seroepidemiological studies of visceral leishmaniasis in Iran."},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879\u2010020\u201005558\u20107"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vprsr.2017.03.010"},{"issue":"4","key":"e_1_2_11_16_1","first-page":"75","article-title":"Canine visceral leishmaniasis in Boyer Ahmad district, Kohgiluyeh & Boyer Ahmad province, southwest of Iran","volume":"7","author":"Moshfe A.","year":"2012","journal-title":"Iranian journal of parasitology"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2009.07.004"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.432"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.07.004"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vcp.12413"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2019.08.014"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2020.104591"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2020.101604"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1134\/S1022795420060125"}],"container-title":["Veterinary Medicine and Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.846","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/vms3.846","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/vms3.846","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T04:26:56Z","timestamp":1692678416000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/vms3.846"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,27]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1002\/vms3.846"],"URL":"https:\/\/doi.org\/10.1002\/vms3.846","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/VMS3.846\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v3\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v3\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v1\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v3\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v2\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/VMS3.846\/v1\/review1","asserted-by":"object"}]},"ISSN":["2053-1095","2053-1095"],"issn-type":[{"value":"2053-1095","type":"print"},{"value":"2053-1095","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,27]]},"assertion":[{"value":"2022-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}