{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:31:06Z","timestamp":1790148666809,"version":"4.0.1"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T00:00:00Z","timestamp":1657843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Hepatocellular carcinoma (HCC) is the fifth most diagnosed cancer and the second leading cause of cancer\u2010related deaths worldwide. Sorafenib is the standard treatment used in the advanced stages of HCC. Cell therapy with mesenchymal stem cells (MSCs)\u2010based cell therapy has proven effective in immune regulation and tumour growth inhibition.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>In this study, we investigated the anti\u2010inflammatory effect of MSCs on HCC xenografts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human HepG2 cell lines were subcutaneously implanted into the flank of 12 nude mice, divided into three groups: the control group, the IV group (intravenous MSCs injection) and the local group (local MSCs injection). Mice were sacrificed 6 weeks after tumour implantation, and tumours were resected entirety. Quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) measured the gene expression of inflammatory markers, including tumour necrosis factor\u2010\u03b1 (TNF\u2010\u03b1), interleukin (IL)\u20101\u03b1 and IL\u201010. Aspartate transaminase (AST), alanine transaminase (ALT) and urea levels were measured using spectrophotometry to ensure the safety of MSC therapy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Gene expressions for all three inflammatory markers were reduced in both MSCs groups compared to the control group. AST, ALT and urea levels remained in normal ranges.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>MSC therapy can reduce inflammation in HCC xenograft mouse models.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.886","type":"journal-article","created":{"date-parts":[[2022,7,15]],"date-time":"2022-07-15T11:28:27Z","timestamp":1657884507000},"page":"2086-2091","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti\u2010inflammatory effect of mesenchymal stem cells on hepatocellular carcinoma in the xenograft mice model"],"prefix":"10.1002","volume":"8","author":[{"given":"Saieh","family":"Hajighasemlou","sequence":"first","affiliation":[{"name":"Department of Tissue Engineering &amp; Applied Cell Sciences Tehran University of Medical Sciences (TUMS)  Tehran Iran"},{"name":"Food and Drug Administration Ministry of Health and Medical Education  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohsen","family":"Nikbakht","sequence":"additional","affiliation":[{"name":"Hematology, Oncology &amp; Stem Cell Transplantation Research Center Tehran University of Medical Sciences (TUMS)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeedreza","family":"Pakzad","sequence":"additional","affiliation":[{"name":"Food and Drug Administration Ministry of Health and Medical Education  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdolnaser","family":"Azadbakht","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, Central Tehran Branch Islamic Azad university  Tehran Iran"},{"name":"Stem cells Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch Islamic Azad university  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samad","family":"Muhammadnejad","sequence":"additional","affiliation":[{"name":"Gene Therapy Research Center, Digestive Diseases Research Institute Tehran University of Medical Sciences  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milad","family":"Mirmoghtadaei","sequence":"additional","affiliation":[{"name":"Immunology, Asthma and Allergy Research Institute Tehran University of Medical Sciences (TUMS)  Tehran Iran"},{"name":"Children's Medical Center, Pediatrics Center of Excellence Tehran University of Medical Sciences (TUMS)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safoora","family":"Gharibzadeh","sequence":"additional","affiliation":[{"name":"Department of Epidemiology and Biostatistics Research Centre for Emerging and Reemerging Infectious Diseases Pasteur institute  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iman","family":"Seyhoun","sequence":"additional","affiliation":[{"name":"Department of Tissue Engineering &amp; Applied Cell Sciences Tehran University of Medical Sciences (TUMS)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javad","family":"Verdi","sequence":"additional","affiliation":[{"name":"Department of Tissue Engineering &amp; Applied Cell Sciences Tehran University of Medical Sciences (TUMS)  Tehran Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,15]]},"reference":[{"issue":"3","key":"e_1_2_12_2_1","first-page":"2135","article-title":"Cytokine expression in colon carcinoma","volume":"25","author":"Baier P. 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