{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T03:35:46Z","timestamp":1778038546289,"version":"3.51.4"},"reference-count":38,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Neospora<\/jats:italic> <jats:italic>caninum<\/jats:italic> has been documented to infect most domestic wildlife but is known to primarily infect dogs and cattle and is considered an important cause of abortion in camels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objective<\/jats:title><jats:p>The aim of this study was to estimate the molecular detection of <jats:italic>Neospora caninum<\/jats:italic> in tissues of naturally infected camelids.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Brain, tongue (bottom and tip) and masseter muscles from 35 slaughtered camelids from Tataouine and M\u00e9denine regions were collected (<jats:italic>n<\/jats:italic> = 140 samples). PCR was used to amplify and detect <jats:italic>N. caninum<\/jats:italic> DNA in tissues samples followed by sequencing of some PCR products. A phylogenetic tree was then constructed to compare the partial sequences of the ITS1 gene with GenBank sequences. Histopathology examination was used to detect <jats:italic>Neospora spp<\/jats:italic>. cysts, but no lesions were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The overall molecular detection of <jats:italic>N. caninum<\/jats:italic> in camelids was 34.3% (12\/35).<\/jats:p><jats:p>The highest molecular detection of <jats:italic>N. caninum<\/jats:italic> was recorded in animals of more than 3 years old (6\/9) and in animals aged between 1 and 3 years old (4\/12). Whilst, the lowest molecular detection (2\/14) was observed in animals 1 year or younger (<jats:italic>p<\/jats:italic> = 0.035).<\/jats:p><jats:p>There were no significant differences in molecular detection of <jats:italic>N. caninum<\/jats:italic> according to both locality and gender (<jats:italic>p<\/jats:italic> &gt; 0.05). Similarly, there was no difference of prevalence between different anatomical locations. Comparison of the partial sequences of the ITS1 gene revealed 100\u201395.5% similarity among our <jats:italic>N. caninum<\/jats:italic> amplicon (MW551566) and those deposited in GenBank.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results highlight the presence of a risk infection by <jats:italic>N. caninum<\/jats:italic> in camels. For preventing <jats:italic>N. caninum<\/jats:italic> infection further studies are needed to improve our knowledge about the epidemiology of neosporosis in North Africa.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.901","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T09:41:49Z","timestamp":1660642909000},"page":"2241-2247","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First molecular detection of <i>Neospora caninum<\/i> from naturally infected slaughtered camels in Tunisia"],"prefix":"10.1002","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7831-6211","authenticated-orcid":false,"given":"Yosra","family":"Amdouni","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie University Manouba Institution de la Recherche et de l'Enseignement Sup\u00e9rieur Agricoles \u00c9cole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire de Sidi Thabet  Sidi Thabet Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imen","family":"abedennebi","sequence":"additional","affiliation":[{"name":"Laboratoire d'anatomie Pathologique \u00c9cole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire de Sidi Thabet  Sidi Thabet Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9919-7522","authenticated-orcid":false,"given":"Safa","family":"Amairia","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie University Manouba Institution de la Recherche et de l'Enseignement Sup\u00e9rieur Agricoles \u00c9cole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire de Sidi Thabet  Sidi Thabet Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amara","family":"Abdelkader","sequence":"additional","affiliation":[{"name":"Laboratoire d'anatomie Pathologique \u00c9cole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire de Sidi Thabet  Sidi Thabet Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Walid","family":"Chandoul","sequence":"additional","affiliation":[{"name":"Ministry of Agriculture Water Resources and Maritime Fisheries, Arrondissement de Production Animale de M\u00e9denine  M\u00e9denine Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7074-2021","authenticated-orcid":false,"given":"Mohamed","family":"Gharbi","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie University Manouba Institution de la Recherche et de l'Enseignement Sup\u00e9rieur Agricoles \u00c9cole Nationale de M\u00e9decine V\u00e9t\u00e9rinaire de Sidi Thabet  Sidi Thabet Tunisia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,16]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.21608\/bvmj.2017.30466"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2013.04.008"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/tbed.12828"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1515\/ap\u20102018\u20100083"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.2478\/s11686\u2010019\u201000105\u20100"},{"key":"e_1_2_11_7_1","first-page":"197","volume-title":"Statistique \u00e9pid\u00e9miologie","author":"Ancelle T.","year":"2006"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010017\u20105470\u20106"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109975(07)80003\u2010X"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE\u2010260R"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2015.04.002"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020\u20107519(99)00134\u20104"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.3347\/kjp.2003.41.1.1"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2011.05.031"},{"key":"e_1_2_11_15_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1201\/9781315152561-5","volume-title":"Neosporosis in animals","author":"Dubey J. 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