{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:27:57Z","timestamp":1790224077398,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T00:00:00Z","timestamp":1663545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2018YFD0500802"],"award-info":[{"award-number":["2018YFD0500802"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2018NZDZX0006"],"award-info":[{"award-number":["2018NZDZX0006"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2021ZDZX0010"],"award-info":[{"award-number":["2021ZDZX0010"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2020YFN0147"],"award-info":[{"award-number":["2020YFN0147"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>It has been reported Interferon\u2010\u03bb (IFN\u2010\u03bb) has stronger antiviral effect than other interferons. IFN\u2010\u03bb can induce antiviral interferon stimulated genes (ISGs) in epithelia to protect against virus. Pseudorabies virus (PRV) infection in pigs resulting in fatal encephalitis in newborn piglets, respiratory disorders in finishing pigs, reproductive disorders in sows and other symptoms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Since the effect of IFN\u2010\u03bb on inhibiting PRV proliferation is still unknown. Inthis study, we investigate the relative contribution of porcine IFN\u2010\u03bb3 toward controlling the infection of PRV in vitro. Our findings may provide a new insight for the prevention and treatment of PRV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Therefore, the antiviral assay, western blot, qRT\u2010PCR and ELISA assay were used to investigating the contribution of IFN\u2010\u03bb against PRV in PK\u201015 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Here, we demonstrate that the replication of PRV in PK\u201015 cells was inhibited after pre\u2010treatment with IFN\u2010\u03bb3, and such inhibition was dose dependent. Overexpression of IFN\u2010\u03bb3 receptor (IFNLR) also restricted virus titre in PK\u201015 cells. In addition, IFN\u2010\u03bb3 also increased the mRNA and protein expression of interferon\u2010stimulated genes 15 (ISG15), 2\u2032\u20105\u2032\u2010oligoadenylate synthase 1 (OAS1), IFN\u2010inducible transmembrane 3 (IFITM3) and myxoma resistance protein 1 (Mx1) in PRV\u2010infected PK\u201015 cells. Other than modulation ISGs, IFN\u2010\u03bb specifically activated IFN\u2010\u03b3 mRNA expression not IFN\u2010\u03b1 or IFN\u2010\u03b2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>IFN\u2010\u03bb3 had antiviral activity against PRV and the upregulation of ISGs and IFN\u2010\u03b3 mRNA expression may be the mechanism of IFN\u2010\u03bb3's antiviral activities. Thus, IFN\u2010\u03bb3 has a decisive function that greatly limits PRV replication in PK\u201015 cells. Our study explores the antiviral activity of IFN\u2010\u03bb3 on PRV for the first time.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.933","type":"journal-article","created":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T19:40:03Z","timestamp":1663616403000},"page":"2444-2450","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Investigation of the anti\u2010pseudorabies virus activity of interferon lambda 3 in cultured porcine kidney epithelial cells"],"prefix":"10.1002","volume":"8","author":[{"given":"Huidan","family":"Deng","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Sichuan Agricultural University  Cheng Du Sichuan Province China"},{"name":"Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province Sichuan Agriculture University  Wenjiang Chengdu 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