{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T16:08:35Z","timestamp":1787674115857,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T00:00:00Z","timestamp":1663804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003121","name":"Ferdowsi University of Mashhad","doi-asserted-by":"publisher","award":["3\/48891"],"award-info":[{"award-number":["3\/48891"]}],"id":[{"id":"10.13039\/501100003121","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Optimal amount of vitamin D for the proper functioning of the immune system is different from the required vitamin D amount for bones to prevent rickets. However, reports on vitamin D reference values in dogs are minimal, and there is still not enough information regarding the relationship between vitamin D and various variables such as disease, age, breed, diet type, and so on, as well as its relationship with haematological and serum biochemical parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Obgectives<\/jats:title>\n                    <jats:p>The present study aimed to determine reference values of 25(OH) Vit D in dogs and its concentration in different groups, categorized based on age, sex, breed, housing conditions, and diet, as well as 25(OH) Vit D relationship with hematology and serum biochemistry parameters.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this study, 90 healthy dogs were selected to determine the reference value of 25 (OH) Vit D of serum after evaluating of their haematological and biochemical parameters to assess their general health. Dogs were divided into different groups according to above\u2010mentioned variables. Serum 25 (OH) Vit D was subsequently measured by the ELISA method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The median concentration of 25 (OH) Vit D was 52.50\u00a0ng\/ml with minimum and maximum amounts of 14.00 and 155.57\u00a0ng\/ml, respectively. No significant difference was observed between 25 (OH) Vit D levels in the studied dogs regarding their different age, sex, breed, diet, housing condition, and reproductive status. Serum 25 (OH) Vit D concentration is directly correlated with the number of band neutrophils (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). We also witnessed indirect correlations between serum 25 (OH) Vit D levels and the number of blood eosinophils and serum glucose (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In the present study age, sex, breed, housing condition and age had no significant effects on the amounts of 25(OH) vitamin D. According to correlations of vitamin D with MCH, band and eosinophil numbers and glucose, vitamin D may have a role in erythropoiesis and leukocytes response and also in energy metabolism in dog.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.943","type":"journal-article","created":{"date-parts":[[2022,9,22]],"date-time":"2022-09-22T17:09:10Z","timestamp":1663866550000},"page":"2360-2366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The effects of age, sex, breed, diet, reproductive status and housing condition on the amounts of 25(OH) vitamin D in the serum of healthy dogs: Reference values"],"prefix":"10.1002","volume":"8","author":[{"given":"Kimia","family":"Alizadeh","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Clinical Sciences Ferdowsi University of Mashhad  Mashhad Iran"},{"name":"Department of Diagnostic Medicine &amp; Pathobiology College of Veterinary Medicine Kansas State University  Manhattan Kansas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saba","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Clinical Sciences Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4856-6244","authenticated-orcid":false,"given":"Ali Asghar","family":"Sarchahi","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Clinical Sciences Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3756-8890","authenticated-orcid":false,"given":"Mehrdad","family":"Mohri","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Department of Clinical Sciences Ferdowsi University of Mashhad  Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,9,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(02)00789-0"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2013.08.060"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"1","article-title":"Calcitriol increases burst forming unit\u2010erythroid (BFU\u2010E) in vitro proliferation in chronic uremia. 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