{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T00:32:22Z","timestamp":1773102742086,"version":"3.50.1"},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T00:00:00Z","timestamp":1663718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2020M682569"],"award-info":[{"award-number":["2020M682569"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp; Sci"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Tigecycline is regarded as a last\u2010resort antimicrobial agent against multidrug resistance (MDR). However, resistance\u2010nodulation\u2010division efflux pump resistance genes, such as <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic>, that confer resistance to tigecycline have emerged.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>This study aimed to characterise the <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic> gene cluster in <jats:italic>Pseudomonas juntendi<\/jats:italic> and <jats:italic>Proteus terrae<\/jats:italic> isolated from a pig farm.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Samples were obtained from a Chinese piggery and included 92 pig faecal samples, 56 wastewater samples, 23 drinking water samples, 28 sow vagina samples and nine sow uterus swabs. The presence of <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic> was detected using a polymerase chain reaction assay, and the antimicrobial susceptibility profile of the <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic>\u2010positive isolates was determined using the broth dilution method. Genomic locations were identified using whole\u2010genome sequencing and bioinformatics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified two <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic>\u2010positive isolates, and both isolates were highly resistant to antibiotics such as tigecycline. In addition, we identified several mobile elements (IS<jats:italic>Pa7<\/jats:italic>, IS<jats:italic>Cfr1<\/jats:italic>, IS<jats:italic>Vsa3<\/jats:italic>) and insertion sequences (Tn<jats:italic>As2<\/jats:italic>, Tn<jats:italic>As3<\/jats:italic>) in <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic>\u2010positive isolates that could increase the potential for the spread of MDR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>To the best of our knowledge, this study is the first to report the detection of <jats:italic>tmexCD3\u2010toprJ3<\/jats:italic> in <jats:italic>P. juntendi<\/jats:italic> and <jats:italic>P. terrae<\/jats:italic> isolated from a pig farm.<\/jats:p><\/jats:sec>","DOI":"10.1002\/vms3.950","type":"journal-article","created":{"date-parts":[[2022,9,21]],"date-time":"2022-09-21T19:38:41Z","timestamp":1663789121000},"page":"2460-2465","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Emergence of the resistance\u2010nodulation\u2010division efflux pump <i>tmexCD3\u2010toprJ3<\/i> gene confers resistance to tigecycline in <i>Pseudomonas juntendi<\/i> and <i>Proteus terrae<\/i> isolated from a pig farm in China"],"prefix":"10.1002","volume":"8","author":[{"given":"Jie","family":"Yang","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine Hunan Agricultural University  Changsha Hunan China"},{"name":"Hunan Engineering Technology Research Center of Veterinary Drugs Hunan Agricultural University  Changsha Hunan 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