{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T18:28:21Z","timestamp":1790792901376,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T00:00:00Z","timestamp":1667520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Veterinary Medicine &amp;amp; Sci"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Babesia<\/jats:italic>\n                      and\n                      <jats:italic>Theileria<\/jats:italic>\n                      are potential threats to the livestock industry, causing considerable economic losses. These tick\u2010borne blood parasites are more prevalent in crossbred cattle than local cattle in Bangladesh.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To confirm the species of\n                      <jats:italic>Babesia<\/jats:italic>\n                      and\n                      <jats:italic>Theileria<\/jats:italic>\n                      in crossbred cattle from the northern part of Bangladesh using conventional and molecular tools.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A total of 385 crossbred cattle blood samples were subjected to DNA extraction and PCR. For molecular detection,\n                      <jats:italic>B. bigemina<\/jats:italic>\n                      rhoptry\u2010associated protein 1a,\n                      <jats:italic>B. bovis<\/jats:italic>\n                      spherical body protein\u20104, and\n                      <jats:italic>Theileria<\/jats:italic>\n                      spp. 18S rRNA were used as the marker genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Using PCR, only 72 (18.7%) samples were found piroplasm positive, of which 12.2%\n                      <jats:italic>Theileria<\/jats:italic>\n                      , 4.7%\n                      <jats:italic>Babesia<\/jats:italic>\n                      , and 1.8% mixed infections. Both\n                      <jats:italic>Babesia<\/jats:italic>\n                      (7.3%),\n                      <jats:italic>Theileria<\/jats:italic>\n                      (7.7%) and mixed (2.8%) infections were detected in Sirajganj, and only\n                      <jats:italic>Theileria<\/jats:italic>\n                      (20.4%) was detected in Rangpur district. By PCR and nPCR we detected\n                      <jats:italic>B. bigemina<\/jats:italic>\n                      and\n                      <jats:italic>T. annulata<\/jats:italic>\n                      in Sirajganj district, and\n                      <jats:italic>Theileria<\/jats:italic>\n                      sp. in Rangpur district. The target gene sequences of isolated pathogens confirmed\n                      <jats:italic>B. bigemina<\/jats:italic>\n                      and\n                      <jats:italic>T. annulata<\/jats:italic>\n                      , and\n                      <jats:italic>Theileria<\/jats:italic>\n                      sp from these samples. Blood smears of all samples were also examined microscopically for\n                      <jats:italic>Babesia<\/jats:italic>\n                      and\/or\n                      <jats:italic>Theileria<\/jats:italic>\n                      spp. and 14.3% of samples were found positive, of which 5.9%\n                      <jats:italic>Babesia<\/jats:italic>\n                      and 8.3%\n                      <jats:italic>Theileria<\/jats:italic>\n                      . Generally, the pathogens detected in Sirajgang and Rangpur were genetically related to South Asia, particularly South East Asian isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings provide information for a better understanding of the epidemiology of\n                      <jats:italic>Babesia<\/jats:italic>\n                      and\n                      <jats:italic>Theileria<\/jats:italic>\n                      as well as to improve the approaches for diagnosis and control of tick\u2010borne diseases in Bangladesh.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/vms3.989","type":"journal-article","created":{"date-parts":[[2022,11,4]],"date-time":"2022-11-04T13:33:29Z","timestamp":1667568809000},"page":"899-906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Molecular detection of\n                    <i>Babesia<\/i>\n                    and\n                    <i>Theileria<\/i>\n                    from crossbred cattle in Sirajganj and Rangpur districts of Bangladesh"],"prefix":"10.1002","volume":"9","author":[{"given":"Md. Jakir","family":"Hossain","sequence":"first","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanjana","family":"Raut","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul Pratap","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8315-0463","authenticated-orcid":false,"given":"Pravin","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6176-1036","authenticated-orcid":false,"given":"Md. Shahadat","family":"Hossain","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5462-7982","authenticated-orcid":false,"given":"Anita Rani","family":"Dey","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajran","family":"Kabir","sequence":"additional","affiliation":[{"name":"Department of Microbiology &amp; Hygiene Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7350-3093","authenticated-orcid":false,"family":"Anisuzzaman","sequence":"additional","affiliation":[{"name":"Department of Parasitology Bangladesh Agricultural University  Mymensingh Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9538-2520","authenticated-orcid":false,"given":"Md. 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