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PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) was used in this study to predict the metagenomic potentials and characterize the microbial community structure and functional features in anammox aggregates (e.g., sludge flocs, biofilms, and granules). The results showed that\n                      <jats:italic>Candidatus Brocadia<\/jats:italic>\n                      was the most dominant anammox genera in all aggregates (38.0% in flocs, 69.4% in biofilm, and 52.0% in granules) and the functional gene involved in the anammox process was detected in the highest amount in biofilms, followed by granules and flocs. Furthermore, the anammox microbial aggregation pathway was explored that anammox bacteria have strong motility and high capability for early attachment. Anammox bacteria could produce large amounts of EPS (extracellular polymeric substances) regulated by quinolone and transport to extracellular environment through type II secretion system. The strong ability of c\u2010di\u2010GMP (bis\u2010(3\u2032\u20105\u2032)\u2010cyclic dimeric guanosine monophosphate) synthesis enabled a stable architectural structure of aggregation. This study elucidated the aggregation mechanism of anammox microorganisms at the genetic level to enhance the stability of anammox processes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Practitioner points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>\n                            <jats:italic>Candidatus Brocadia<\/jats:italic>\n                            was the most dominant anammox genera in aggregates.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Anammox bacteria have strong motility and high attachment capability.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Anammox bacteria possess strong EPS synthesis regulated by quinolone.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>c\u2010di\u2010GMP synthesis enables a stable structure of aggregation.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/wer.1529","type":"journal-article","created":{"date-parts":[[2021,2,6]],"date-time":"2021-02-06T02:52:19Z","timestamp":1612579939000},"page":"2549-2558","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":54,"title":["Metagenomic prediction analysis of microbial aggregation in anammox\u2010dominated community"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0415-9460","authenticated-orcid":false,"given":"Fangxu","family":"Jia","sequence":"first","affiliation":[{"name":"Beijing International Scientific and Technological Cooperation Base of Water Pollution Control Techniques for Antibiotics and Resistance Genes School of Civil Engineering Beijing Jiaotong University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongzhen","family":"Peng","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology Engineering Research Center of Beijing Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianwei","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology Engineering Research Center of Beijing Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyao","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology Engineering Research Center of Beijing Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering Beijing University of Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3652-0951","authenticated-orcid":false,"given":"Hong","family":"Yao","sequence":"additional","affiliation":[{"name":"Beijing International Scientific and Technological Cooperation Base of Water Pollution Control Techniques for Antibiotics and Resistance Genes School of Civil Engineering Beijing Jiaotong University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,2,22]]},"reference":[{"key":"e_1_2_7_2_1","article-title":"Standard methods for the examination of water and waste water","author":"Association A. 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