{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:21:40Z","timestamp":1790882500566,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T00:00:00Z","timestamp":1631059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100010980","name":"Nitte Meenakshi Institute of Technology","doi-asserted-by":"publisher","award":["NUFR3\/2017\/06\/03"],"award-info":[{"award-number":["NUFR3\/2017\/06\/03"]}],"id":[{"id":"10.13039\/501100010980","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Water Environment Research"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:sec><jats:title>Abstract<\/jats:title><jats:p>The study aimed to screen for the presence of multidrug\u2010resistant <jats:styled-content style=\"fixed-case\"><jats:italic>Klebsiella pneumoniae<\/jats:italic><\/jats:styled-content> from diarrheal stool and environmental water samples and to check the epidemiological link between the two categories. Isolates obtained after culturing on different media were tested for antibiotic resistance and extended\u2010spectrum beta\u2010lactamase (ESBL) production. Polymerase chain reaction (PCR) analysis was done for important \u03b2\u2010lactamase encoding genes. Random amplified polymorphic DNA (RAPD) typing was done using two primers. Results showed a high prevalence of <jats:styled-content style=\"fixed-case\"><jats:italic>K.\u00a0pneumoniae<\/jats:italic><\/jats:styled-content> from fish market effluents compared with stool and well water. Stool isolates showed high resistance to ceftazidime (80.0%) and cefepime (80.0%), fish market effluent isolates to cefoperazone\u2013sulbactam (92.1%), and erythromycin (78.9%), while well water isolates to erythromycin (72.7%) and cefuroxime (54.4%). The ESBL genes <jats:italic>bla<\/jats:italic><jats:sub>CTX<\/jats:sub>, <jats:italic>bla<\/jats:italic><jats:sub>SHV<\/jats:sub>, and <jats:italic>bla<\/jats:italic><jats:sub>TEM<\/jats:sub> were detected in 22.85%, 14.28%, and 42.85% of <jats:styled-content style=\"fixed-case\"><jats:italic>K.\u00a0pneumoniae<\/jats:italic><\/jats:styled-content> isolates, respectively. The results of RAPD\u2010PCR showed high genetic similarities between the isolates from different sources.<\/jats:p><\/jats:sec><jats:sec><jats:title>Practitioner Points<\/jats:title><jats:p>\n<jats:list list-type=\"bullet\">\n<jats:list-item><jats:p>Detection of multi\u2010drug resistant <jats:italic>Klebsiella<\/jats:italic> strains in hospital wastewater and drinking water sources has progressively increased since its emerging resistance to third\u2010generation cephalosporins and carbapenems.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Detection of beta\u2010lactamase encoding genes by molecular techniques and typing by random amplified polymorphic DNA (RAPD) can be useful in identifying the genetic fingerprints for epidemiological study.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Implementation of effective antimicrobial stewardship program and infection control policy thereby helps assess the risk factors associated with infections.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/wer.1630","type":"journal-article","created":{"date-parts":[[2021,8,25]],"date-time":"2021-08-25T23:00:02Z","timestamp":1629932402000},"page":"2740-2753","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Antibiotic resistance and random amplified polymorphic DNA typing of <scp><i>Klebsiella pneumoniae<\/i><\/scp> isolated from clinical and water samples"],"prefix":"10.1002","volume":"93","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8098-4868","authenticated-orcid":false,"given":"Shobha","family":"Giri","sequence":"first","affiliation":[{"name":"Department of Microbiology KS Hegde Medical Academy, NITTE (Deemed to be University)  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malathi","family":"Shekar","sequence":"additional","affiliation":[{"name":"Department of Aquatic animal health management College of Fisheries  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9522-1185","authenticated-orcid":false,"given":"A. Veena","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Microbiology KS Hegde Medical Academy, NITTE (Deemed to be University)  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puneeth T.","family":"G","sequence":"additional","affiliation":[{"name":"Department of Aquatic animal health management College of Fisheries  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash K.","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Pediatrics and Office of Global Health Wake Forest School of Medicine and Brenner Children's Hospital  Winston\u2010Salem North Carolina USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,9,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2012.03203.x"},{"key":"e_1_2_9_3_1","volume-title":"Current protocols in molecular biology","author":"Ausubel F.","year":"1995"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/45.4_ts.493"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.04981-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00211"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2018.04.005"},{"key":"e_1_2_9_8_1","unstructured":"Clinical and Laboratory Standards Institute. 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