{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T18:57:10Z","timestamp":1785524230250,"version":"3.56.0"},"reference-count":52,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T00:00:00Z","timestamp":1771113600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,1]],"date-time":"2026-02-01T00:00:00Z","timestamp":1769904000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Water Environment Research"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Mainstream anaerobic ammonium oxidation (ANAMMOX) offers a promising alternative to conventional nitrogen removal processes in municipal wastewater treatment. However, research has largely focused on its potential to reduce oxygen demand rather than external carbon requirements. This study compared the external carbon demand of mainstream ANAMMOX with that of anaerobic\u2013anoxic\u2013oxic (A2O) and partial denitrification\/ANAMMOX (PD\/A) processes. Stoichiometric ratios, validated in a laboratory\u2010scale packed\u2010bed biofilm reactor, were implemented in a simulation framework reflecting representative conditions in Korean wastewater treatment plants. A 5\u2009\u00d7\u20095\u2009\u00d7\u20093\u2009\u00d7\u20093 simulation matrix evaluated external carbon demand and its sensitivity to sidestream ammonium concentration (SAC), mainstream ammonium concentration (MAC), mainstream total organic carbon concentration (MTC), and sidestream flow rate (SFR) through gradient\u2010based analysis and regression modeling. Mainstream ANAMMOX reduced external carbon demand by up to ~10.8%\u201372.2% compared with A2O and ~3.7%\u201341.2% compared with PD\/A, depending on influent loading conditions. SAC and MTC were identified as the dominant drivers of carbon reduction. Notably, higher MAC levels slightly diminished the relative carbon savings due to the increased external carbon requirement for polishing the stoichiometric nitrate byproduct. Three composite indices (sidestream effective nitrogen load index, total effective nitrogen load index, and sidestream contribution ratio [SCR]) were proposed, with SCR exhibiting the highest predictive accuracy. Although the absolute savings are site\u2010specific, these findings suggest that mainstream ANAMMOX can substantially decrease reliance on external carbon sources, and the proposed indices provide practical tools for process evaluation and design optimization.<\/jats:p>","DOI":"10.1002\/wer.70301","type":"journal-article","created":{"date-parts":[[2026,2,16]],"date-time":"2026-02-16T03:16:34Z","timestamp":1771211794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Practical Indices for Minimizing External Carbon Demand in Mainstream ANAMMOX: A Sensitivity\u2010 and Regression\u2010Based Approach"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-6751-9132","authenticated-orcid":false,"given":"Sungryul","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Civil Engineering Seoul National University of Science and Technology  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6097-0250","authenticated-orcid":false,"given":"Kyungik","family":"Gil","sequence":"additional","affiliation":[{"name":"Department of Civil Engineering Seoul National University of Science and Technology  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2022.114510"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2015.04.041"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/09593330.2017.1385860"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/09593330.2015.1133711"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2018.09.312"},{"key":"e_1_2_10_7_1","volume-title":"Water and Wastewater Engineering","author":"Choi E.","year":"2001"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2013.092"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2017.03.016"},{"issue":"3","key":"e_1_2_10_10_1","first-page":"351","article-title":"Nitrogen Removal Processes\u2014II. 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