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We investigated the anaerobic digestion (\n                    <jats:sc>AD<\/jats:sc>\n                    ) of raw sewage WW (RW) and EF for biohydrogen and biomethane production using a microbial consortia designed from complementary natural wastes selected for their distinct microbial complexity and buffering capacity. The consortia design included sequential mixing, heat\u2010pretreatment, organic load shock, and anaerobic acclimatization for selective enrichment of fermentative, hydrogen\u2010producing, and methanogenic communities. Three different heat\u2010pretreatment consortia and their untreated counterparts were tested in two\n                    <jats:sc>AD<\/jats:sc>\n                    batch experiments (B1 and B3). Biogas production, gas compositions, and microbial dynamics were explored using gas chromatography and 16S rRNA gene sequencing. B3 reactors produced more biogas than B1 and controls, highlighting enhanced microbial activity after acclimatization. For RW, the heat\u2010pretreated inoculum (R.C2Tx) yielded highest cumulative gas (81\u2009mL) with 37% biomethane, attributed to enrichment of fermentative and hydrogenotrophic taxa. In contrast, the untreated consortia (E.C2UTx) performed better for EF (76.7\u2009mL total biogas, 12.7% biomethane, and 5.8% biohydrogen), highlighting the enrichment of microbes with ability for degradation of Fe\u2010rich, complex substrates. Enrichment of\n                    <jats:italic>Proteobacteria<\/jats:italic>\n                    ,\n                    <jats:italic>Pseudomonas<\/jats:italic>\n                    , and\n                    <jats:italic>Methanobacterium<\/jats:italic>\n                    in most B3 samples correlated with higher gas yield.\n                  <\/jats:p>","DOI":"10.1002\/wer.70425","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T12:25:08Z","timestamp":1779971108000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Microbial Dynamics During the Anaerobic Digestion of Raw Sewage and Electro\u2010Flocculated Wastewater for Biohydrogen and Biomethane Production using Synthetically Designed Microbial Consortia"],"prefix":"10.1002","volume":"98","author":[{"given":"Rashmi","family":"Ira","sequence":"first","affiliation":[{"name":"School of Biosciences and Bioengineering Indian Institute of Technology, Mandi  Mandi Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vikas","family":"Sharma","sequence":"additional","affiliation":[{"name":"School of Biosciences and Bioengineering Indian Institute of Technology, Mandi  Mandi Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shrawan","family":"Kumar","sequence":"additional","affiliation":[{"name":"School of Biosciences and Bioengineering Indian Institute of Technology, Mandi  Mandi Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lalita","family":"Sharma","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences Indian Institute of Technology, Mandi  Mandi Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0399-9705","authenticated-orcid":false,"given":"Aditi","family":"Halder","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences Indian Institute of Technology, Mandi  Mandi Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9270-8775","authenticated-orcid":false,"given":"Tulika","family":"Prakash","sequence":"additional","affiliation":[{"name":"School of Biosciences and Bioengineering Indian Institute of Technology, Mandi  Mandi Himachal Pradesh India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.12911\/22998993\/147835"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40974-023-00280-x"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2022.e01238"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12649-018-00558-w"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijhydene.2015.08.106"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.1c05258"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00174850"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-010-2788-8"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0209-9"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2018.02.092"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49313-8"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1177\/0734242X241252906"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9039-9"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00570\u201023"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.4c02789"},{"key":"e_1_2_12_17_1","first-page":"1","volume-title":"Bergey's Manual of Systematics of Archaea and Bacteria","author":"Dedysh S. 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