{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:38:45Z","timestamp":1792463925176,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Water Environment Research"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Although integrated wastewater\u2010fed fisheries in the Ramsar\u2010designated East Kolkata Wetlands (EKW) provide important ecosystem services, the long\u2010term spatial and temporal dynamics of microbial attenuation in these managed pond systems remain insufficiently characterized. This study assessed the spatiotemporal dynamics of chlorophyll\u2010a concentration and culturable bacterial abundance along a canal\u2010to\u2010pond transition system over 22\u2009months. Water samples were collected from four fixed locations during 22 sampling months between September 2023 and August 2025 to determine total viable count (TVC), chlorophyll\u2010a concentration, and chlorophyll\u2010a\u2010based trophic state index (TSI). TVC decreased by 79.86%, from a geometric mean of 22,409 colony\u2010forming units (CFU\/mL) at the canal source to 4513\u2009CFU\/mL at the distal pond site, and differed significantly among sampling locations (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.0001). TVC was also significantly higher during the dry season than during the wet season (Mann\u2013Whitney\n                    <jats:italic>U<\/jats:italic>\n                    \u2009=\u20091244.5,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.017). Chlorophyll\u2010a concentrations did not differ significantly among sampling locations (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.078), although the mean concentration was 82.8% higher across the three pond stations than at the canal source. Mean TSI values ranged from 47.1 to 52.3 across the sampled gradient. Predictive modeling with Analysis of covariance (ANCOVA) showed that chlorophyll\u2010a was not a significant predictor of log10\u2010transformed TVC after accounting for sampling location (\n                    <jats:italic>F<\/jats:italic>\n                    \u2009=\u20090.48,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009=\u20090.491). These results suggest that site\u2010associated differences, rather than a statistically detectable chlorophyll\u2010a effect, are associated with microbial decline within the sampled gradient. Because other physical, chemical, hydraulic, and biological factors were not measured, the observed spatial association cannot be attributed to location alone.\n                  <\/jats:p>","DOI":"10.1002\/wer.70630","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:43:11Z","timestamp":1792460591000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Decoupling Algal Biomass and Microbial Attenuation in Wastewater\u2010Fed Fisheries: A Spatiotemporal Analysis in an Indian Ramsar Site"],"prefix":"10.1002","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3298-0643","authenticated-orcid":false,"given":"Santanu","family":"Roy","sequence":"first","affiliation":[{"name":"Department of Life Sciences Presidency University  Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8003-3065","authenticated-orcid":false,"given":"Agniswar","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"Centre for Distributed Computing Jadavpur University  Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3810-0887","authenticated-orcid":false,"given":"Toufique","family":"Shahnowaz","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Presidency University  Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4712-5460","authenticated-orcid":false,"given":"Partha Pratim","family":"Mondal","sequence":"additional","affiliation":[{"name":"Department of Zoology Government General Degree College  Mohanpur West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3417-4530","authenticated-orcid":false,"given":"Souryadeep","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Presidency University  Kolkata West Bengal India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,19]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envc.2021.100158"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2022.154176"},{"key":"e_1_2_13_4_1","unstructured":"Bureau of Indian Standards.1991. \u201cSpecifications for Surface Water Discharge (No. (IS\u20102296)).\u201d Bureau of Indian Standards. New Delhi."},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1977.22.2.0361"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.22207\/JPAM.14.3.38"},{"key":"e_1_2_13_7_1","unstructured":"EKWMA andWISA.2021. \u201cEast Kolkata Wetlands: Management Action Plan 2021\u20132026\u201d."},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.5455\/javar.2022.i639"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41597\u2010024\u201003506\u20107"},{"key":"e_1_2_13_10_1","volume-title":"The Reuse Opportunity: Wastewater Report","author":"International Water Association","year":"2018"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015\u20103796(17)30778\u20103"},{"key":"e_1_2_13_12_1","doi-asserted-by":"crossref","first-page":"1293","DOI":"10.1007\/978-90-481-9659-3_222","volume-title":"The Wetland Book: I: Structure and Function, Management, and Methods","author":"Kumar R.","year":"2018"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.679743"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13157\u2010021\u201001509\u20103"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40808\u2010016\u20100136\u20104"},{"key":"e_1_2_13_16_1","volume-title":"Current Perspectives on Irrigation and Drainage","author":"Pham D. D.","year":"2017"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.49.1.1\u20107.1985"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.4172\/2155\u20106199.1000211"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2021.223"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.2166\/wst.2009.673"},{"issue":"3","key":"e_1_2_13_21_1","first-page":"47","article-title":"Planktonic Cyanoprokaryota and Bacillariophyta of East Kolkata Wetlands Ecosystem, a Ramsar Site of India With Reference to Diversity and Taxonomic Study","volume":"6","author":"Singha Roy A.","year":"2015","journal-title":"Journal of Algal Biomass Utilization"},{"key":"e_1_2_13_22_1","volume-title":"Fisheries Research Board of Canada Bulletin","author":"Strickland J. D. H.","year":"1972"},{"key":"e_1_2_13_23_1","unstructured":"Tacoli C. andG.McGranahan.2015. \u201cUrbanization Rural\u2013Urban Migration and Urban Poverty.\u201d World Migration Report. International Institute for Environment and Development. London."},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10661\u2010021\u201009679\u20107"},{"key":"e_1_2_13_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356\u2010021\u201013756\u20102"},{"key":"e_1_2_13_26_1","unstructured":"United Nations.2018. \u201cSustainable Cities Human Mobility and International Migration: A Concise Report.\u201d United Nations. New York."},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.1080\/00856401.2017.1341038"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph16101739"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecolind.2023.110029"}],"container-title":["Water Environment Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/wer.70630","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/wer.70630","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/wer.70630","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T01:43:19Z","timestamp":1792460599000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/wer.70630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10]]},"references-count":28,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1002\/wer.70630"],"URL":"https:\/\/doi.org\/10.1002\/wer.70630","archive":["Portico"],"relation":{},"ISSN":["1061-4303","1554-7531"],"issn-type":[{"value":"1061-4303","type":"print"},{"value":"1554-7531","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10]]},"assertion":[{"value":"2026-05-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70630"}}