{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T00:41:31Z","timestamp":1793925691841,"version":"4.8.0"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T00:00:00Z","timestamp":1676246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81725004"],"award-info":[{"award-number":["81725004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870703"],"award-info":[{"award-number":["81870703"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82271138"],"award-info":[{"award-number":["82271138"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["World j. otorhinolaryngol.-head neck surg."],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To analyze the oxidative stress status and its association with tissue neutrophilia and oral steroid response in chronic rhinosinusitis with nasal polyps (CRSwNP) patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The levels of total oxidant status (TOS) were detected in the sinonasal tissues by using specific assay kits. Tissue neutrophil was examined by immunohistochemical staining, and oxidant status index (OSI) was evaluated in polyps tissues, and the messenger RNA\u00a0(mRNA) levels of superoxide dismutase 2\u00a0(SOD2), aldehyde dehydrogenase 1\u00a0(ALDH1A1), and microsomal glutathione S\u2010transferase 1 (MGST1) were examined using quantitative real\u2010time polymerase chain reaction\u00a0in the sinonasal tissues. The receiver operating characteristics (ROCs) curve of ALDH1A1, MGST1, and SOD2 mRNA levels were evaluated to determine the steroid response of CRSwNP patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The levels of TOS and OSI were significantly higher in CRSwNP and CRSsNP than in normal controls, and OSI in polyps tissues was positively associated with tissue neutrophilia and poor steroid response. The ALDH1A1, MGST1, and SOD2 mRNA levels showed comparable accuracy as predictors of poor steroid response indicated by the\u00a0area under the curve.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings provided evidence that the increased level of oxidative stress contributes to enhanced tissue neutrophilia and poor steroid response in CRSwNP patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/wjo2.91","type":"journal-article","created":{"date-parts":[[2023,2,13]],"date-time":"2023-02-13T08:36:05Z","timestamp":1676277365000},"page":"320-327","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Enhanced oxidative stress is associated with tissue neutrophilia and poor steroid response in chronic rhinosinusitis with nasal polyps"],"prefix":"10.1002","volume":"9","author":[{"given":"Xian\u2010Ting","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bai\u2010Wen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Jie","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2477-2879","authenticated-orcid":false,"given":"Hua\u2010Bin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De\u2010Hui","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Affiliated Eye and ENT Hospital Fudan University Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,2,13]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhino12.000"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2011.07.049"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2020.03.007"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2012.01.079"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anl.2019.09.011"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.793073"},{"key":"e_1_2_11_8_1","first-page":"173","article-title":"Subepithelial neutrophil infiltration as a predictor of the surgical outcome of chronic rhinosinusitis with nasal polyps","volume":"59","author":"Kim DK","year":"2020","journal-title":"Rhinology"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pnpbp.2013.02.015"},{"key":"e_1_2_11_10_1","first-page":"1341","article-title":"Quantitative analysis of SOD2, ALDH1A1 and MGST1 messenger ribonucleic acid in anterior lens epithelium of patients with pseudoexfoliation syndrome","volume":"19","author":"Strzalka\u2010Mrozik B","year":"2013","journal-title":"Mol Vis"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/jop.2000.16.193"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2011.02769.x"},{"key":"e_1_2_11_13_1","first-page":"248","article-title":"Evaluation of total oxidative stress parameters in patients with nasal polyps","volume":"33","author":"Bozkus F","year":"2013","journal-title":"Acta Otorhinolaryngol Ital"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.2500\/ajra.2014.28.3963"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-017-0679-0"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.4193\/Rhino11.249"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.12.972"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2020.12.1.1"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2011.03715.x"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0000000000000106"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11356-016-6653-x"}],"container-title":["World Journal of Otorhinolaryngology - 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