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Additionally, the stability of endotoxin ensures it cannot be removed efficiently through conventional sterilization techniques such as autoclaving and ionizing radiation. In recent times, the true significance of this hurdle has come to light with multiple reports from the United States Nanotechnology Characterization Laboratory, in particular, along with our own experiences of endotoxin testing from multiple Horizon 2020\u2010funded projects which highlight the importance of this issue for the clinical translation of nanomaterials. Herein, we provide an overview on the topic of endotoxin contamination of nanomaterials intended for biomedical applications.<\/jats:p>\n                  <jats:p>\n                    This article is categorized under:\n                    <jats:list list-type=\"simple\">\n                      <jats:list-item>\n                        <jats:p>Therapeutic Approaches and Drug Discovery &gt; Emerging Technologies<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>Toxicology and Regulatory Issues in Nanomedicine &gt; Regulatory and Policy Issues in Nanomedicine<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1002\/wnan.1738","type":"journal-article","created":{"date-parts":[[2021,7,13]],"date-time":"2021-07-13T00:07:46Z","timestamp":1626134866000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Endotoxin contamination of engineered nanomaterials: Overcoming the hurdles associated with endotoxin testing"],"prefix":"10.1002","volume":"13","author":[{"given":"Gary","family":"Hannon","sequence":"first","affiliation":[{"name":"Nanomedicine and Molecular Imaging Group, Department of Clinical Medicine Trinity Translational Medicine Institute  Dublin Ireland"},{"name":"Laboratory of Biological Characterization of Advanced Materials (LBCAM), Trinity Translational Medicine Institute, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4371-2214","authenticated-orcid":false,"given":"Adriele","family":"Prina\u2010Mello","sequence":"additional","affiliation":[{"name":"Nanomedicine and Molecular Imaging Group, Department of Clinical Medicine Trinity Translational Medicine Institute  Dublin Ireland"},{"name":"Laboratory of Biological Characterization of Advanced Materials (LBCAM), Trinity Translational Medicine Institute, Trinity College Dublin  Dublin Ireland"},{"name":"Advanced Materials and Bioengineering Research (AMBER) Centre, CRANN institute, Trinity College Dublin  Dublin Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,7,12]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.000510"},{"key":"e_1_2_15_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.418"},{"key":"e_1_2_15_4_1","doi-asserted-by":"publisher","DOI":"10.1086\/BBLv225n3p137"},{"key":"e_1_2_15_5_1","doi-asserted-by":"publisher","DOI":"10.1177\/0968051907085986"},{"key":"e_1_2_15_6_1","doi-asserted-by":"publisher","DOI":"10.5731\/pdajpst.2017.007849"},{"key":"e_1_2_15_7_1","doi-asserted-by":"publisher","DOI":"10.14573\/altex.1709221"},{"key":"e_1_2_15_8_1","doi-asserted-by":"publisher","DOI":"10.4155\/tde-2018-0076"},{"key":"e_1_2_15_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2266"},{"key":"e_1_2_15_10_1","doi-asserted-by":"publisher","DOI":"10.14573\/altex.2003241"},{"key":"e_1_2_15_11_1","doi-asserted-by":"publisher","DOI":"10.1089\/sur.2012.155"},{"key":"e_1_2_15_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2013.04.009"},{"key":"e_1_2_15_13_1","first-page":"3","article-title":"The treatment of malignant tumors by repeated inoculations of erysipelas. 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