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Across these systems, multivalent RNA\u2010protein interactions promote self\u2010organization, contributing directly to liquid\u2013liquid phase separation (LLPS) in nucleoli and nuclear speckles, while facilitating assembly and intermediate states in mature structurally organized vRNPs. This review critically examines the molecular grammar underlying these three paradigmatic systems, focusing on how sequence\u2010encoded RNA\u2010IDR chemistry determines condensate morphology, compositional selectivity, and material properties. We then evaluate the experimental and computational approaches used to characterize RNA\u2010protein assemblies, with particular focus on coarse\u2010grained (CG) forcefield development across HPS, Mpipi, CALVADOS, and 1BPA families. We also discuss how emerging multi\u2010domain protein (MDP) models and RNA\u2010compatible forcefields are optimizing the predictive accuracy of molecular simulations. Finally, we explore physics\u2010based and data\u2010driven predictors of LLPS, and reiterate the need for a framework that can predict sequence\u2010based heterotypic IDR\u2010RNA co\u2010phase separation.<\/jats:p>","DOI":"10.1002\/wrna.70061","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:51:09Z","timestamp":1790949069000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<scp>RNA<\/scp>\n                    \u2010Protein Assemblies: A Review of Biophysical Principles and Coarse\u2010Grained Modeling Approaches"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8479-2054","authenticated-orcid":false,"given":"Muhammad Waleed","family":"Iqbal","sequence":"first","affiliation":[{"name":"Zernike Institute for Advanced Materials University of Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5632-9727","authenticated-orcid":false,"given":"Patrick R.","family":"Onck","sequence":"additional","affiliation":[{"name":"Zernike Institute for Advanced Materials University of Groningen  Groningen the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"crossref","unstructured":"Acharya B. 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