{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:37:03Z","timestamp":1791283023192,"version":"4.1.0"},"reference-count":117,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2011,4,25]],"date-time":"2011-04-25T00:00:00Z","timestamp":1303689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["WIREs RNA"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Virtually, all eukaryotic mRNAs are synthesized as precursor molecules that need to be extensively processed in order to serve as a blueprint for proteins. The three most prevalent processing steps are the capping reaction at the 5\u2032\u2010end, the removal of intervening sequences by splicing, and the formation of poly (A)\u2010tails at the 3\u2032\u2010end of the message by polyadenylation. A large number of proteins and small nuclear ribonucleoprotein complexes (snRNPs) interact with the mRNA and enable the different maturation steps. This chapter focuses on the biogenesis of snRNPs, the major components of the pre\u2010mRNA splicing machinery (spliceosome). A large body of evidence has revealed an intricate and segmented pathway for the formation of snRNPs that involves nucleo\u2010cytoplasmic transport events and elaborates assembly strategies. We summarize the knowledge about the different steps with an emphasis on\n                    <jats:italic>trans<\/jats:italic>\n                    \u2010acting factors of snRNP maturation of higher eukaryotes.\n                    <jats:italic>WIREs RNA<\/jats:italic>\n                    2011 2 718\u2013731 DOI: 10.1002\/wrna.87\n                  <\/jats:p>\n                  <jats:p>\n                    This article is categorized under:\n                    <jats:list list-type=\"explicit-label\">\n                      <jats:list-item>\n                        <jats:p>RNA Interactions with Proteins and Other Molecules &gt; RNA\u2013Protein Complexes<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>RNA Processing &gt; Splicing Mechanisms<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>RNA Export and Localization &gt; Nuclear Export\/Import<\/jats:p>\n                      <\/jats:list-item>\n                      <jats:list-item>\n                        <jats:p>RNA in Disease and Development &gt; RNA in Disease<\/jats:p>\n                      <\/jats:list-item>\n                    <\/jats:list>\n                  <\/jats:p>","DOI":"10.1002\/wrna.87","type":"journal-article","created":{"date-parts":[[2011,4,25]],"date-time":"2011-04-25T13:24:22Z","timestamp":1303737862000},"page":"718-731","source":"Crossref","is-referenced-by-count":141,"title":["Biogenesis of spliceosomal small nuclear 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