{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:54:44Z","timestamp":1761490484430,"version":"3.41.2"},"reference-count":106,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T00:00:00Z","timestamp":1519689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002996","name":"Hartstichting","doi-asserted-by":"publisher","award":["CVON2014\u201040 DOSIS","CVON2015\u201012 eDETECT"],"award-info":[{"award-number":["CVON2014\u201040 DOSIS","CVON2015\u201012 eDETECT"]}],"id":[{"id":"10.13039\/501100002996","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008955","name":"Wilhelmina Kinderziekenhuis","doi-asserted-by":"publisher","award":["OZF\/14"],"award-info":[{"award-number":["OZF\/14"]}],"id":[{"id":"10.13039\/501100008955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["WIREs Mechanisms of Disease"],"published-print":{"date-parts":[[2018,7]]},"abstract":"<jats:p>Dilated cardiomyopathy (DCM) is a form of severe failure of cardiac muscle caused by a long list of etiologies ranging from myocardial infarction, DNA mutations in cardiac genes, to toxics. Systems analysis integrating next\u2010generation sequencing (NGS)\u2010based omics approaches, such as the sequencing of DNA, RNA, and chromatin, provide valuable insights into DCM mechanisms. The outcome and interpretation of NGS methods can be affected by the localization of cardiac biopsy, level of tissue degradation, and variable ratios of different cell populations, especially in the presence of fibrosis. Heart tissue composition may even differ between sexes, or siblings carrying the same disease causing mutation. Therefore, before planning any experiments, it is important to fully appreciate the complexities of DCM, and the selection of samples suitable for given research question should be an interdisciplinary effort involving clinicians and biologists. The list of NGS omics datasets in DCM to date is short. More studies have to be performed to contribute to public data repositories and facilitate systems analysis. In addition, proper data integration is a difficult task requiring complex computational approaches. Despite these complications, there are multiple promising implications of systems analysis in DCM. By combining various types of datasets, for example, RNA\u2010seq, ChIP\u2010seq, or 4C, deep insights into cardiac biology, and possible biomarkers and treatment targets, can be gained. Systems analysis can also facilitate the annotation of noncoding mutations in cardiac\u2010specific DNA regulatory regions that play a substantial role in maintaining the tissue\u2010 and cell\u2010specific transcriptional programs in the heart.<\/jats:p><jats:p>This article is categorized under:\n<jats:list list-type=\"simple\">\n<jats:list-item><jats:p>Physiology &gt; Mammalian Physiology in Health and Disease<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Laboratory Methods and Technologies &gt; Genetic\/Genomic Methods<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Laboratory Methods and Technologies &gt; RNA Methods<\/jats:p><\/jats:list-item>\n<\/jats:list>\n<\/jats:p>","DOI":"10.1002\/wsbm.1419","type":"journal-article","created":{"date-parts":[[2018,2,27]],"date-time":"2018-02-27T13:22:47Z","timestamp":1519737767000},"source":"Crossref","is-referenced-by-count":12,"title":["Systems analysis of dilated cardiomyopathy in the next generation sequencing era"],"prefix":"10.1002","volume":"10","author":[{"given":"Magdalena","family":"Harakalova","sequence":"first","affiliation":[{"name":"Department of Cardiology, Division Heart and Lungs University Medical Center Utrecht, Utrecht University  Utrecht Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Folkert W.","family":"Asselbergs","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Division Heart and Lungs University Medical Center Utrecht, Utrecht University  Utrecht Netherlands"},{"name":"Durrer Center for Cardiovascular Research Netherlands Heart Institute  Utrecht Netherlands"},{"name":"Institute of Cardiovascular Science University College London  London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,27]]},"reference":[{"key":"e_1_2_10_1_2_1","unstructured":"ENCODE. 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