{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:34:11Z","timestamp":1760121251317},"reference-count":30,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T00:00:00Z","timestamp":1101254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although yeast are generally non\u2010haemolytic, we have found that addition of alcohol vapour confers haemolytic properties on many strains of yeast and other fungi. We have called this phenomenon \u2018microbial alcohol\u2010conferred haemolysis\u2019 (MACH). MACH is species\u2010 and strain\u2010specific: whereas all six <jats:italic>Candida tropicalis<\/jats:italic> strains tested were haemolytic in the presence of ethanol, none among 10 <jats:italic>C. glabrata<\/jats:italic> strains tested exhibited this phenomenon. Among 27 <jats:italic>C. albicans<\/jats:italic> strains and 11 <jats:italic>Saccharomyces cerevisiae<\/jats:italic> strains tested, ethanol\u2010mediated haemolysis was observed in 11 and 4 strains, respectively. Haemolysis is also dependent on the alcohol moiety: <jats:italic>n<\/jats:italic>\u2010butanol and <jats:italic>n<\/jats:italic>\u2010pentanol could also confer haemolysis, whereas methanol and 2\u2010propanol did not. Haemolysis was found to be dependent on initial oxidation of the alcohol. Reduced haemolysis was observed in specific alcohol dehydrogenase mutants of both <jats:italic>Aspergillus nidulans<\/jats:italic> and <jats:italic>S. cerevisiae<\/jats:italic>. MACH was not observed during anaerobic growth, and was reduced in the presence of pararosaniline, an aldehyde scavenger. Results suggest that initial oxidation of the alcohol to the corresponding aldehyde is an essential step in the observed phenomenon. Copyright \u00a9 2004 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1183","type":"journal-article","created":{"date-parts":[[2004,11,24]],"date-time":"2004-11-24T16:46:38Z","timestamp":1101314798000},"page":"1335-1342","source":"Crossref","is-referenced-by-count":22,"title":["Alcohol\u2010mediated haemolysis in yeast"],"prefix":"10.1002","volume":"21","author":[{"given":"Amir","family":"Shuster","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nir","family":"Osherov","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mel","family":"Rosenberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,11,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2001.tb00570.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1021"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10408419109113511"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.184.16.4400-4408.2002"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.6.2591-2597.1987"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.64.5.1109"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0165-1218(91)90128-9"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1996.5301061.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M005769200"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3640025"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-2920.2001.00235.x"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/18.9.1739"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/21.4.663"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.01849.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/8.10.1543"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(87)90339-2"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.8.2971-2974.2001"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"5154","DOI":"10.1128\/iai.62.11.5154-5156.1994","article-title":"Production of a haemolytic factor by Candida albicans","volume":"62","author":"Manns JM","year":"1994","journal-title":"Infect Immun"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0215(20001101)88:3<342::AID-IJC4>3.0.CO;2-I"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M311550200"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6918(03)00035-0"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-76-18653"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1530-0277.1999.tb04364.x"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4439(00)00086-7"},{"key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"689","DOI":"10.1099\/13500872-145-3-689","article-title":"Characterization of a haemolytic factor from Candida albicans","volume":"145","author":"Watanabe T","year":"1999","journal-title":"Microbiology"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1979.tb13260.x"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5429.901"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202411200"},{"key":"e_1_2_6_31_1","first-page":"1375","article-title":"Ethanol tolerance and the induction of stress proteins by ethanol in Candida albicans","volume":"134","author":"Zeuthen ML","year":"1981","journal-title":"J Gen Microbiol"}],"container-title":["Yeast"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fyea.1183","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/yea.1183","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T23:22:54Z","timestamp":1697498574000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/yea.1183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11,24]]},"references-count":30,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1002\/yea.1183"],"URL":"https:\/\/doi.org\/10.1002\/yea.1183","archive":["Portico"],"relation":{},"ISSN":["0749-503X","1097-0061"],"issn-type":[{"value":"0749-503X","type":"print"},{"value":"1097-0061","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11,24]]}}}