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Nevertheless, information concerning the physiological role of this enzyme, a distant homologue of mammalian 3\u2010\u03b2\u2010hydroxysteroid dehydrogenases, is scarce. Combining quantitative phenotypic profiling and protein expression analysis studies, we here report the involvement of yeast Gre2p in ergosterol metabolism. Growth was significantly and exclusively reduced in <jats:italic>gre2<\/jats:italic>\u0394 strains subjected to environmental stress straining the cell membrane. Furthermore, whereas no compensatory mechanisms were activated due to loss of Gre2p during growth in favourable conditions (synthetic defined media, no stress), a striking and highly specific induction of the ergosterol biosynthesis pathway, represented by the enzymes Erg10p, Erg19p and Erg6p, was observed in <jats:italic>gre2<\/jats:italic>\u0394 during growth in a stress condition in which lack of Gre2p significantly affects growth. Involvement of Gre2p in ergosterol metabolism was confirmed by application of an array of selective inhibitors of lipid biosynthesis, as <jats:italic>gre2<\/jats:italic>\u0394 displayed vastly impaired tolerance exclusively to agents targeting the ergosterol biosynthesis. The approach outlined here, combining broad\u2010spectrum phenotypic profiling, expression analysis during conditions reducing the growth of the mutant and functional confirmation by application of highly selective inhibitors, may prove a valuable tool in gene functional analysis. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1363","type":"journal-article","created":{"date-parts":[[2006,4,5]],"date-time":"2006-04-05T14:50:25Z","timestamp":1144248625000},"page":"389-398","source":"Crossref","is-referenced-by-count":28,"title":["Involvement of yeast <i>YOL151W<\/i>\/<i>GRE2<\/i> in ergosterol metabolism"],"prefix":"10.1002","volume":"23","author":[{"given":"Jonas","family":"Warringer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Blomberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,4,5]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)49767-6"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.5.1255-1265.2000"},{"key":"e_1_2_5_4_1","first-page":"1175","volume-title":"Goodman and 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