{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:12:17Z","timestamp":1789909937164,"version":"4.0.1"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T00:00:00Z","timestamp":1156118400000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The fission yeast <jats:italic>Schizosaccharomyces pombe<\/jats:italic> is an excellent model organism for cell biology. However, its genetic toolbox is less developed than that of <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. In the first part of this study we describe an improved inducible expression vector based on tetracycline regulation of the CaMV35S promoter, which is also capable of chromosomal integration and therefore works in minimal and in rich media. We found that anhydrotetracycline is a superior ligand for induction. Maximum expression levels were observed after 12 h in minimal media (EMM) and after 9 h in rich media (YES), which is faster than the <jats:italic>nmt1<\/jats:italic> promoter system. The system was combined with a convenient recombineering\u2010based subcloning strategy for ease of cloning. In the second part we present four template plasmids, pSVEM\u2010bsd, pSVEM\u2010nat, pSVEM\u2010kan and pSVEM\u2010hph, which harbour four recyclable disruption cassettes based on the Cre recombinase lox71\/66 strategy for use in PCR targeting methods. Cre\u2010mediated excision leaves a non\u2010functional mutant lox site in the genome, allowing the reiterative usage of these cassettes for multiple targetings. These cassettes are also configured with dual eukaryotic\/prokaryotic promoters so that they can be used for recombineering in <jats:italic>E. coli<\/jats:italic>. Amongst other purposes, this permits the rapid and convenient creation of targeting constructs with much longer homology arms for difficult and complex targetings in the <jats:italic>Sz. pombe<\/jats:italic> genome. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1396","type":"journal-article","created":{"date-parts":[[2006,8,21]],"date-time":"2006-08-21T11:33:00Z","timestamp":1156159980000},"page":"813-823","source":"Crossref","is-referenced-by-count":39,"title":["Recombineering reagents for improved inducible expression and selection marker re\u2010use in <i>Schizosaccharomyces pombe<\/i>"],"prefix":"10.1002","volume":"23","author":[{"given":"Axel","family":"Erler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello","family":"Maresca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. 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