{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T12:25:44Z","timestamp":1697545544163},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,12,18]],"date-time":"2006-12-18T00:00:00Z","timestamp":1166400000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We report the cloning of a <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> cDNA, here named <jats:italic>Pb<\/jats:italic>Cnx, encoding the homologue of the endoplasmic reticulum molecular chaperone calnexin. Calnexin specifically recognizes monoglucosylated glycoproteins in the endoplasmic reticulum, thus being an essential component of the complex that interacts with the folded state of nascent secreted glycoproteins. The <jats:italic>Pb<\/jats:italic>Cnx open reading frame was found in a 1701 base pair (bp) fragment that encodes a 567 amino acid protein with an estimated mass of 62 680 Da. Northern and Southern blot hybridizations showed that <jats:italic>Pb<\/jats:italic>Cnx is encoded by a single, or a low number of, gene copies. <jats:italic>Pb<\/jats:italic>Cnx contains the hallmark KPEDWD motifs that are found in all members of the calnexin\/calreticulin family proteins. A cDNA\u2010encoding <jats:italic>Pb<\/jats:italic>Cnx was overexpressed as recombinant protein in <jats:italic>Escherichia coli.<\/jats:italic> The purified recombinant <jats:italic>Pb<\/jats:italic>Cnx was recognized by 6 out of 10 sera from PCM patients, a result that rules out its possible consideration for further use in diagnosis. Using confocal microscopy with anti\u2010<jats:italic>Pb<\/jats:italic>Cnx mouse serum against yeast forms, a cytoplasmic staining pattern was observed. Copyright \u00a9 2006 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1438","type":"journal-article","created":{"date-parts":[[2006,12,18]],"date-time":"2006-12-18T10:51:24Z","timestamp":1166439084000},"page":"79-87","source":"Crossref","is-referenced-by-count":2,"title":["Cloning, characterization and expression of a calnexin homologue from the pathogenic fungus <i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1002","volume":"24","author":[{"given":"Luciano","family":"dos Santos Feitosa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9lia Maria","family":"de Almeida Soares","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e1rcia Regina Machado","family":"dos Santos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre Melo","family":"Bail\u00e3o","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia","family":"Xander","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renato Arruda","family":"Mortara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Daniel","family":"Lopes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,18]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.1.217"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"21458","DOI":"10.1016\/S0021-9258(18)54661-5","article-title":"Expression of calreticulin in Escherichia coli and identification of its Ca2+ \u2010binding domain","volume":"266","author":"Baksh S","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.5.2.379-390.2006"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.2002.00752.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"2969","DOI":"10.1016\/S0021-9258(18)53868-0","article-title":"Purification of a 90\u2010kDa protein (Band VII) from cardiac sarcoplasmic reticulum. 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