{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T06:56:20Z","timestamp":1761807380753},"reference-count":50,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2007,12,20]],"date-time":"2007-12-20T00:00:00Z","timestamp":1198108800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2008,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The dimorphic fungus <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> is the causative agent of the most frequent systemic mycosis in Latin America. In humans, infection starts by inhalation of fungal propagules, which reach the pulmonary epithelium and differentiate into the yeast parasitic phase. Here we describe the characterization of a Dfg5p (<jats:styled-content>d<\/jats:styled-content>efective for <jats:styled-content>f<\/jats:styled-content>ilamentous <jats:styled-content>g<\/jats:styled-content>rowth) homologue of <jats:italic>P. brasiliensis<\/jats:italic>, a predictable cell wall protein, first identified in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>. The protein, the cDNA and genomic sequences were analysed. The cloned cDNA was expressed in <jats:italic>Escherichia coli<\/jats:italic> and the purified r<jats:italic>Pb<\/jats:italic>Dfg5p was used to obtain polyclonal antibodies. Immunoelectron microscopy and biochemical studies demonstrated the presence of <jats:italic>Pb<\/jats:italic>Dfg5p in the fungal cell wall. Enzymatic treatments identified <jats:italic>Pb<\/jats:italic>Dfg5p as a \u03b2\u2010glucan linked protein that undergoes <jats:italic>N<\/jats:italic>\u2010glycosylation. The r<jats:italic>Pb<\/jats:italic>Dfg5p bound to extracellular matrix components, indicating that those interactions could be important for initial steps leading to <jats:italic>P. brasiliensis<\/jats:italic> attachment and colonization of host tissues. The <jats:italic>P. brasiliensis dfg5<\/jats:italic> nucleotide and deduced protein, <jats:italic>Pb<\/jats:italic>Dfg5p, sequences reported in this paper had been submitted to the GenBank database under Accession Nos AY307855 (cDNA) and DQ534495 (genomic). Copyright \u00a9 2007 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1574","type":"journal-article","created":{"date-parts":[[2007,12,20]],"date-time":"2007-12-20T16:25:56Z","timestamp":1198167956000},"page":"141-154","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of <i>Paracoccidioides brasiliensis Pb<\/i>Dfg5p, a cell\u2010wall protein implicated in filamentous growth"],"prefix":"10.1002","volume":"25","author":[{"given":"Nadya","family":"da Silva Castro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f4nica Santiago","family":"Barbosa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zilma Alves","family":"Maia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia 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