{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:13:03Z","timestamp":1776258783820,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,11,10]],"date-time":"2009-11-10T00:00:00Z","timestamp":1257811200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa de S\u00e3o Paulo (FAPESP)","award":["06\/60642-2"],"award-info":[{"award-number":["06\/60642-2"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa de S\u00e3o Paulo (FAPESP)","award":["07\/55731-9"],"award-info":[{"award-number":["07\/55731-9"]}]},{"name":"Postdoctoral Fellowship","award":["06\/50694-5"],"award-info":[{"award-number":["06\/50694-5"]}]},{"DOI":"10.13039\/501100008353","name":"CNPq and FAEPA","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008353","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Yeast"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The dimorphic fungus <jats:italic>Paracoccidioides brasiliensis<\/jats:italic> is the causative agent of paracoccidioidomycosis, the most frequent systemic mycosis in Latin America. Our group has been working with paracoccin, a <jats:italic>P. brasiliensis<\/jats:italic> lectin with MM 70 kDa, which is purified by affinity with immobilized <jats:italic>N<\/jats:italic>\u2010acetylglucosamine (GlcNAc). Paracoccin has been described to play a role in fungal adhesion to extracellular matrix components and to induce high and persistent levels of TNF\u03b1 and nitric oxide production by macrophages. In the cell wall, paracoccin colocalizes with the \u03b2\u20101,4\u2010homopolymer of GlcNAc into the budding sites of the <jats:italic>P. brasiliensis<\/jats:italic> yeast cell. In this paper we present a protocol for the chitin\u2010affinity purification of paracoccin. This procedure provided higher yields than those achieved by means of the technique based on the affinity of this lectin with GlcNAc and had an impact on downstream assays. SDS\u2013PAGE and Western blot analysis revealed similarities between the <jats:italic>N<\/jats:italic>\u2010acetylglucosamine\u2010 and chitin\u2010bound fractions, confirmed by MALDI\u2013TOF\u2013MS of trypsinic peptides. Western blot of two\u2010dimensional gel electrophoresis of the yeast extract showed a major spot with <jats:italic>M<\/jats:italic><jats:sub>r<\/jats:sub> 70 000 and pI approximately 5.63. Morevover, an <jats:italic>N<\/jats:italic>\u2010acetyl\u2010\u03b2\u2010<jats:sc>D<\/jats:sc>\u2010glucosaminidase activity was reported for paracoccin, thereby providing new insights into the mechanisms that lead to cell wall remodelling and opening new perspectives for its structural characterization. Copyright \u00a9 2009 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1731","type":"journal-article","created":{"date-parts":[[2009,11,11]],"date-time":"2009-11-11T03:30:29Z","timestamp":1257910229000},"page":"67-76","source":"Crossref","is-referenced-by-count":21,"title":["Paracoccin from <i>Paracoccidioides brasiliensis<\/i>; purification through affinity with chitin and identification of <i>N<\/i>\u2010acetyl\u2010\u03b2\u2010<scp>D<\/scp>\u2010glucosaminidase activity"],"prefix":"10.1002","volume":"27","author":[{"given":"Fausto Bruno","family":"dos Reis Almeida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leandro Licursi","family":"de Oliveira","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo Valle","family":"de Sousa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Cristina 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