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To discover the cellular processes that control SO<jats:sub>2<\/jats:sub> and H<jats:sub>2<\/jats:sub>S production, we screened a library of <jats:italic>Saccharomyces cerevisiae<\/jats:italic> deletion mutants. Deletion of 12 genes led to increased H<jats:sub>2<\/jats:sub>S productivity. Ten of these genes are known to be involved in sulfur\u2010containing amino acid metabolism, whereas <jats:italic>UBI4<\/jats:italic> functions in the ubiquitin\u2010proteasome system and <jats:italic>SKP2<\/jats:italic> encodes an F\u2010box\u2010containing protein whose function is unknown. We found that the <jats:italic>skp2<\/jats:italic> mutant accumulated H<jats:sub>2<\/jats:sub>S and SO<jats:sub>2<\/jats:sub>, because the adenosylphophosulfate kinase Met14p is a substrate of SCF<jats:sup>Skp2<\/jats:sup> and more stable in the <jats:italic>skp2<\/jats:italic> mutant than in the wild\u2010type strain. Furthermore, the <jats:italic>skp2<\/jats:italic> mutant grew more slowly than the wild\u2010type strain under nutrient\u2010limited conditions. Metabolome analysis showed that the concentration of intracellular cysteine is lower in the <jats:italic>skp2<\/jats:italic> mutant than in the wild\u2010type strain. The slow growth of the <jats:italic>skp2<\/jats:italic> mutant was due to a lower concentration of intracellular cysteine, because the addition of cysteine suppressed the slow growth. In the <jats:italic>skp2<\/jats:italic> mutant, the cysteine biosynthesis proteins Str2p, Str3p and Str4p are more stable than in the wild\u2010type strain. Moreover, supplementation with methionine, <jats:italic>S<\/jats:italic>\u2010adenosylmethionine, <jats:italic>S<\/jats:italic>\u2010adenosylhomocysteine and homocysteine also suppressed the slow growth. Overexpression of <jats:italic>STR1<\/jats:italic> or <jats:italic>STR4<\/jats:italic> caused a more severe defect in the <jats:italic>skp2<\/jats:italic> mutant. These results suggest that the balance of methionine and cysteine biosynthesis is important for yeast cell growth. Thus, Skp2p is one of the key components regulating this balance and H<jats:sub>2<\/jats:sub>S\/SO<jats:sub>2<\/jats:sub> production. Copyright \u00a9 2010 John Wiley &amp; Sons, Ltd.<\/jats:p>","DOI":"10.1002\/yea.1823","type":"journal-article","created":{"date-parts":[[2010,10,8]],"date-time":"2010-10-08T05:58:27Z","timestamp":1286517507000},"page":"109-121","source":"Crossref","is-referenced-by-count":18,"title":["A novel mechanism regulates H<sub>2<\/sub>S and SO<sub>2<\/sub> production in <i>Saccharomyces 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